Chemistry & Biology
Pseudomonas 4-Quinolones Derive from Fatty Acids
with 20 mM Tris-HCl, pH 7.6. The reaction was incubated for 1 hr at 37ꢀC, and
the production of HHQ was measured by LC/MS, using 4-hydroxy-3-methyl-2-
heptenylquinoline as internal standard (Vial et al., 2008). The assay was per-
formed in duplicate.
Pseudomonas aeruginosa pathogenesis and quorum sensing circuitry regula-
tion: multiple quorum sensing-regulated genes are modulated without
affecting lasRI, rhlRI or the production of N-acyl-L-homoserine lactones.
Mol. Microbiol. 55, 998–1014.
´
Diggle, S.P., Winzer, K., Chhabra, S.R., Worrall, K.E., Camara, M., and
Williams, P. (2003). The Pseudomonas aeruginosa quinolone signal molecule
overcomes the cell density-dependency of the quorum sensing hierarchy,
regulates rhl-dependent genes at the onset of stationary phase and can be
produced in the absence of LasR. Mol. Microbiol. 50, 29–43.
SUPPLEMENTAL INFORMATION
Supplemental Information includes two figures and one table and can be found
Diggle, S.P., Matthijs, S., Wright, V.J., Fletcher, M.P., Chhabra, S.R., Lamont,
I.L., Kong, X., Hider, R.C., Cornelis, P., Ca´ mara, M., and Williams, P. (2007).
The Pseudomonas aeruginosa 4-quinolone signal molecules HHQ and PQS
play multifunctional roles in quorum sensing and iron entrapment. Chem.
Biol. 14, 87–96.
ACKNOWLEDGMENTS
Thanks to Melissa Starkey for providing plasmid pMSR1. This study was sup-
ported by Canadian Institutes of Health Research operating grant MOP-97888
to E.D., a National Sciences and Engineering Research Council of Canada Dis-
covery grant to F.L., and National Institutes of Health grant 2R56AI063433-
06A1 to L.G.R. Respectively, C.E.D. and V.D. were recipients of an MSc and
a PhD scholarship from the Fondation Armand-Frappier. E.D. was a Cher-
Driscoll, J.A., Brody, S.L., and Kollef, M.H. (2007). The epidemiology, patho-
genesis and treatment of Pseudomonas aeruginosa infections. Drugs 67,
351–368.
Farrow, J.M., 3rd, Sund, Z.M., Ellison, M.L., Wade, D.S., Coleman, J.P., and
Pesci, E.C. (2008). PqsE functions independently of PqsR-Pseudomonas
quinolone signal and enhances the rhl quorum-sensing system. J. Bacteriol.
190, 7043–7051.
´
´
cheur-boursier Junior 2 of the Fonds de la Recherche en Sante du Quebec
and holds a Canada Research Chair in Sociomicrobiology.
Received: July 10, 2013
Revised: August 23, 2013
Accepted: September 4, 2013
Published: November 14, 2013
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Galloway, W.R., Hodgkinson, J.T., Bowden, S., Welch, M., and Spring, D.R.
(2012). Applications of small molecule activators and inhibitors of quorum
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