1
.5% w/v CaCO
3
. Adequate aeration was achieved by adhering to
Acknowledgements
a maximal air:culture ratio of 5:1. After 30 min of incubation
◦
We gratefully acknowledge financial support by the Deutsche
Bundesstiftung Umwelt (Contract #13138-32) and by the Eu-
ropean Union within the FP6 funded NoE BioSim (Contract
(
30 C/150 rpm) 2,5-hexanedione was added, resulting in the
stated concentration (standard concentration 1% v/v). Progress
of the biotransformation was monitored by GC. To obtain (5S)-
hydroxy-2-hexanone, biotransformations were stopped after 10 h,
whereas (2S,5S)-hexanediol was obtained after 48 h of reaction.
Cells were removed from the reaction mixture by centrifugation
#
LSHB-CT-2004-005137). Yeast was kindly provided by FALA
Hefe GmbH, Kesselsdorf, Germany. We thank Dr. M. Gruner
and A. Rudolph for recording NMR Spectra. The authors wish to
thank Prof. W.Reschetilowski, Institute of Technical Chemistry,
Dresden University of Technology, for analytical support.
(
5 min 5000g) and extracted three times with acetone. The cell
free supernatant was concentrated in vacuo and subsequently
treated with an excess of acetone in order to precipitate remaining
cell fragments. After filtration acetone extracts were combined,
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Simulations
5
69
Simulation was carried out using software COPASI. Reversible
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max(3→2) = const.) and the resulting maximal concentration of
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This journal is © The Royal Society of Chemistry 2009
and J. J. Heijnen, Biotech. Bioeng., 2000, 69, 370–376.
Org. Biomol. Chem., 2009, 7, 304–314 | 313