FULL PAPERS
Selective Access to All Four Diastereomers of a 1,3-Amino Alcohol
sodium phosphate buffer (100 mM, pH 7.5) containing
0.25 mM PLP and 1M isopropylamine, which was prepared
freshly prior to reaction. After addition of lyophilised
enzyme (5 mgmLÀ1) a stock solution of the amino acceptor
was added to adjust the final concentration to 20 mM of
substrate and 10% (v/v) 2-propanol. After 17 to 18 h of re-
action, the glass vial was shaken without cap for one to two
hours until all substrate was consumed.
Acknowledgements
We thank the “Deutsche Bundesstiftung Umwelt” (grant No.
AZ29937) for financial support and Prof. Byung Gee Kim
(Seoul National University, Seoul, South Korea) for provid-
ing the gene for the Vibrio fluvialis ATA. Furthermore we
would like to thank Dr. Samson Afewerki and Dr. Guangn-
ing Ma, Mid Sweden University, for synthesis of some com-
pounds. KTH Royal Institute of Technology is acknowledged
for an excellence PhD student position to Mattias Anderson.
His contribution is a result of the COST Action CM1303
“Systems Biocatalysis”. Moreover, HK wants to express his
deep gratitude to Philine Pia.
Redox reactions employing KRED from Codexisꢀ: Reac-
tions were performed at 308C in potassium phosphate
buffer (125 mM, pH 7) containing 1.25 mM MgSO4 and
1 mM NADP+, which was prepared freshly prior to the reac-
tion. After addition of lyophilised enzyme (1–2.5 mgmLÀ1),
a stock solution of the substrate in either acetone (for oxida-
tion reactions) or 2-propanol (for reduction reactions) was
used to adjust the final concentration to 10–30 mM substrate
and 10–50% (v/v) co-solvent.
References
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Adv. Synth. Catal. 2015, 357, 1808 – 1814
ꢁ 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
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