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1
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with PBS, confocal images were obtained. From top to bottom:
Green channel: λem = 490-520 nm (λex = 405 nm), Orange chan-
nel: λem = 550-590 nm (λex = 488 nm), Ratio images (RO/G). b) The
corresponding time-dependent arithmetic mean intensity changes
of the obtained images.
(a) Lehmann, A. Neurosci. 1987, 22 (2), 573-578; (b)
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CONCLUSION
In conclusion, we have developed the first fluorescent probe
for Cys metabolism visualization based on the rational design
of the dual recognition sites for Cys and its metabolite SO2,
respectively. The probe featured fast and reversible fluorescent
responses towards Cys which promoted the probe to monitor
the Cys concentration alterations induced by NEM and H2O2
in vitro. Further, with high selectivity towards thiols and SO2,
the probe was successfully applied for exogenous Cys and SO2
imaging in zebrafish. Cellular experiments demonstrated that
the probe could be used for redox dynamic imaging in A549
cells. Importantly, with ratiometric fluorescent responses, the
probe could visualize the enzymatic conversion of Cys to SO2
in living A549 cells which provided a deeper insight into the
physiological processes of Cys. Supported by the present work,
fluorescent probes to image the metabolism of other important
thiols are developing in our lab, and we believe that these
probes will bring a better understanding to the pathological
roles of biothiols.
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ASSOCIATED CONTENT
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Supporting Information. Supplemental figures, synthetic
schemes and experimental procedures, and characterization of all
compounds. This material is available free of charge via the Inter-
AUTHOR INFORMATION
Corresponding Author
*M.X. Meng
*C.X. Yin
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(a) Yu, S.; Yang, X.; Shao, Z.; Feng, Y.; Xi, X.; Shao, R.;
Guo, Q.; Meng, X. Sensor. Actuat. B: Chem. 2016, 235, 362-369; (b)
Yang, X.-F.; Zhao, M.; Wang, G. Sensor. Actuat. B: Chem. 2011, 152
(1), 8-13; (c) Xie, H.; Zeng, F.; Yu, C.; Wu, S. Polym. Chem. 2013, 4
(21), 5416-5424.
Author Contributions
(10) (a) Zhang, Y.; Guan, L.; Yu, H.; Yan, Y.; Du, L.; Liu, Y.;
Sun, M.; Huang, D.; Wang, S. Anal. Chem. 2016, 88 (8), 4426-31; (b)
Yang, X.; Zhou, Y.; Zhang, X.; Yang, S.; Chen, Y.; Guo, J.; Li, X.;
Qing, Z.; Yang, R. Chem. Commun. 2016, 52 (67), 10289-92.
(11) (a) Zhang, Y.; Shao, X.; Wang, Y.; Pan, F.; Kang, R.;
Peng, F.; Huang, Z.; Zhang, W.; Zhao, W. Chem. Commun. 2015, 51
(20), 4245-8; (b) Lee, H. Y.; Choi, Y. P.; Kim, S.; Yoon, T.; Guo, Z.;
Lee, S.; Swamy, K. M.; Kim, G.; Lee, J. Y.; Shin, I.; Yoon, J. Chem.
Commun. 2014, 50 (53), 6967-9; (c) Hong, R.; Han, G.; Fernandez, J.
M.; Kim, B. J.; Forbes, N. S.; Rotello, V. M. J. Am. Chem.Soc. 2006,
128 (4), 1078-9.
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Edu. 1990, 67 (2), 172; (b) Rhee, S. G.; Bae, Y. S.; Lee, S. R.; Kwon,
J. Science's STKE 2000, 2000 (53), pe1; (c) Luo, D.; Smith, S. W.;
Anderson, B. D. J. Pharm. Sci. 2005, 94 (2), 304-16; (d) Gough, D.
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Santamarina, S.; Boronat, S.; Hidalgo, E. Biochemistry 2014, 53 (16),
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§ Fangjun Huo and Yongkang Yue contributed equally.
Notes
The authors declare no competing financial interests.
ACKNOWLEDGMENT
The work was supported by the National Natural Science Founda-
tion of China (No. 21472118, 21672131, 21372005), Talents
Support Program of Shanxi Province (2014401), Shanxi Province
Outstanding Youth Fund (No. 2014021002).
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