K.V. Sashidhara et al. / European Journal of Medicinal Chemistry 50 (2012) 230e235
235
same way to each consecutive row till row B. Row A was kept drug
also thankful to Dr T. K. Chakraborty (Director, CSIR-CDRI) for his
constant support and encouragement. This is CDRI communication
number 8190.
free to serve as positive control and the plate was incubated for 48 h
ꢃ
at 37 C in 5% CO
2
in air. After 72 h of drug exposure, 10
mL/well of
viability marker dye Resazurin or Alamar blue (stock solution
2.5 mg/100 mL PBS) was added and the plate was incubated for
1
Appendix. Supplementary material
another 2e4 h. Fluorimetric reaction was measured using an exci-
tation wavelength of 536 nm and an emission of 588 nm in a fluo-
rimetric plate reader. Data was transferred to Excel and plotted as
per the template using fluorescent signal against corresponding
drug concentration. CC50 values were determined directly.
4
.5.8. Determination of selectivity index
Selectivity index (SI) values of the in vitro active samples
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