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ASSOCIATED CONTENT
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Experimental details including the synthesis of mNADHs,
mNADs, mNADH4s, overꢀexpression of the enzymes,
determination of the molar extinction coefficient for mNADHs,
reductive halfꢀreaction data, steadyꢀstate kinetic data, biocatalytic
transformations, recycling of mNAD+ and crystallization
experiments can be found in the Supporting Information. This
material is available free of charge via the Internet at
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AUTHOR INFORMATION
Corresponding Author
* Nigel.Scrutton@manchester.ac.uk
* F.Hollmann@tudelft.nl
Present Addresses
† Van´t Hoff Institute for Molecular Sciences, University of
Amsterdam, Science Park 904; 1098 XH Amsterdam, The
Netherlands
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Author Contributions
‡ These authors contributed equally.
Notes
The authors declare no competing financial interests.
ACKNOWLEDGMENT
This work was supported by the Marie Curie IEF “Biomimic”
financed by the European Union through the 7th Framework
People Programme (grant agreement number 327647) and by the
UK Biotechnology and Biological Sciences Research Council
(BBSRC; BB/K0017802/1) and GlaxoSmithKline. N.S.S.
received funding as an Engineering and Physical Sciences
Research Council (EPSRC) Established Career Fellow
(EP/J020192/1) and a Royal Society Wolfson Merit Award.
F.G.M received funding as a European Research Council (ERC)
Starting Grant Fellow (H2020, grant agreement 638271,
BioSusAmin). The BBSRC (BB/M017702/1) supported
infrastructure for analytical chemistry. Marc J. F. Strampraad is
gratefully acknowledged for his technical assistance. We thank
Luke Humphreys (GlaxoSmithKline) and Gideon J. Grogan for
fruitful discussions and Diederik J. Opperman for providing
DrOYE and RmOYE. Furthermore, we thank Diamond Light
Source for access to beamlines i02 & i03 (MX8997) that
contributed to the results presented here. The authors thank
Verena Resch for her great artwork on the graphical abstract.
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