2784
M.-C. Cui et al. / Bioorg. Med. Chem. 18 (2010) 2777–2784
ethanol/H2O; 5 min wash in 60% ethanol/H2O; running tap water
for 10 min, and then incubated in PBS (0.2 M, pH 7.4) for 30 min.
The sections were incubated with [125I]10 and [125I]13 in the ab-
sence or in the presence of thioflavin-T (10 mM) for 30 min at room
temperature. The sections were then washed with saturated Li2CO3
in 40% ethanol for 3 min and washed with 40% ethanol for 3 min,
followed by rinsing with water for 30 s. After drying, the 125I-la-
beled sections were exposed to phosphorus film for 2 h then
scanned with the phosphor imaging system (Cyclone, Packard) at
the resolution of 600 dpi. The presence and localization of plaques
were confirmed by the fluorescent staining with thioflavin-S
Supplementary data
Supplementary data associated with this article can be found, in
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[
l
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The authors thank Dr. Lianfeng Zhang (Institute of Laboratory
Animal Science, Chinese Academy of Medical Sciences and Com-
parative Medicine Center of Peking Union Medical College) for
kindly provided the Paraffin-embedded brain sections of AD model
mice, Dr. Hongmei Jia (College of Chemistry, Beijing Normal Uni-
versity) for many thoughtful discussions and preparation of the
manuscript and Dr. Xiaoyan Zhang (College of Life Science, Beijing
Normal University) for assistance in the in vitro neuropathological
staining. The authors also thank Dr. Yan Cheng (Department of
Patho-Functional Bioanalysis, Graduate School of Pharmaceutical
Sciences, Kyoto University) for her help with preparation of the
manuscript. This work was funded by NSFC (20871021).
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