Paper
RSC Advances
18 S. S. Bhattacharya and R. Banerjee, Chemosphere, 2008, 73,
81–85.
Conclusions
19 S. Li, X. Ma, L. Liu and X. Cao, RSC Adv., 2015, 5, 1902–1909.
20 J. Jia, S. Zhang, P. Wang and H. Wang, J. Hazard. Mater.,
2012, 205–206, 150–155.
21 S. Sharma, M. Mukhopadhyay and Z. V. P. Murthy, Sep. Purif.
Rev., 2013, 42, 263–295.
22 Z.-l. Li, J. Nan, J.-q. Yang, X. Jin, A. Katayama and A.-j. Wang,
RSC Adv., 2015, 5, 89157–89163.
23 Y. Wang, X. Chen, J. Liu, F. He and R. Wang, Environ. Sci.
Pollut. Res. Int., 2013, 20, 6222–6231.
24 D. Litthauer, M. J. van Vuuren, A. van Tonder and
F. W. Wolfaardt, Enzyme Microb. Technol., 2007, 40, 563–568.
25 D. Kalyani, S. S. Dhiman, H. Kim, M. Jeya, I. W. Kim and
J. K. Lee, Process Biochem., 2012, 47, 671–678.
26 H. Serrano-Posada, B. Valderrama, V. Stojanoff and
E. Rudino-Pinera, Acta Crystallogr., Sect. F: Struct. Biol.
Cryst. Commun., 2011, 67, 1595–1598.
27 R. J. King, K. A. Short and R. J. Seidler, Appl. Environ.
Microbiol., 1991, 57, 1790–1792.
28 A. Kondo, J. Kohda, Y. Endo, T. Shiromizu, Y. Kurokawa,
K. Nishihara, H. Yanagi, T. Yura and H. Fukuda, J. Biosci.
Bioeng., 2000, 90, 600–606.
We found that laccase from M. ruber (Mrlac) can be properly
folded and synthesized in E. coli under microaerobic conditions
during coexpression with a chaperone protein. Aer the
expression and purication step, its biochemical and kinetic
properties were studied. The spectrophotometric characteriza-
tion (UV-visible and EPR) and sequence alignment of Mrlac
showed that the enzyme has all the ngerprint spectral features
that are characteristic for laccases like MCOs. The puried
Mrlac laccase displayed the ability to oxidize chlorophenol in
a reduced reaction time compared to previously discovered
laccases without the addition of any meditator.
Acknowledgements
This research was partially supported by the Danish National
Advanced Technology Foundation via the B21st technology
platform (Grant no. 001-2011-4). The authors would like to
thank Dr Søren Brander for his help in EPR spectral analysis.
References
29 A. de Marco, Nat. Protoc., 2007, 2, 2632–2639.
1 S. Jones and E. Solomon, Cell. Mol. Life Sci., 2015, 72, 869– 30 M. Goyal and T. K. Chaudhuri, Int. J. Biochem. Cell Biol.,
883.
2015, 64, 277–286.
2 S. Rangabhashiyam, N. Anu and N. Selvaraju, Res. J. Chem. 31 P. Durao, I. Bento, A. T. Fernandes, E. P. Melo, P. F. Lindley
Environ., 2013, 17, 88–95.
and L. O. Martins, J. Biol. Inorg. Chem., 2006, 11, 514–526.
3 P. J. Strong and H. Claus, Crit. Rev. Environ. Sci. Technol., 32 M. Mohammadian, M. Fathi-Roudsari, N. Mollania,
2011, 41, 373–434.
4 T. Kudanga and M. Le Roes-Hill, Appl. Microbiol. Biotechnol.,
2014, 98, 6525–6542.
5 L. Munk, A. Sitarz, D. Kalyani, J. D. Mikkelsen and
A. S. Meyer, Biotechnol. Adv., 2015, 33, 13–24.
6 V. Brissos, L. Pereira, F. D. Munteanu, A. Cavaco-Paulo and
L. O. Martins, Biotechnol. J., 2009, 4, 558–563.
7 P. Sharma, R. Goel and N. Capalash, World J. Microbiol.
Biotechnol., 2007, 23, 823–832.
A. Badoei-Dalfard and K. Khajeh, J. Ind. Microbiol.
Biotechnol., 2010, 37, 863–869.
33 A. E. Palmer, D. W. Randall, F. Xu and E. I. Solomon, J. Am.
Chem. Soc., 1999, 121, 7138–7149.
34 E. Solomon, U. Sundaram and T. Machonkin, Chem. Rev.,
1996, 96, 2563–2605.
35 K. Koschorreck, S. M. Richter, A. B. Ene, E. Roduner,
R. D. Schmid and V. B. Urlacher, Appl. Microbiol.
Biotechnol., 2008, 79, 217–224.
8 S. Brander, J. D. Mikkelsen and K. P. Kepp, PLoS One, 2014, 9, 36 P. Durao, Z. J. Chen, C. S. Silva, C. M. Soares, M. M. Pereira,
e99402.
S. Todorovic, P. Hildebrandt, I. Bento, P. F. Lindley and
9 P. Baldrian, FEMS Microbiol. Rev., 2006, 30, 215–242.
L. O. Martins, Biochem. J., 2008, 412, 339–346.
10 Z. M. Fang, P. Zhou, F. Chang, Q. Yin, W. Fang, J. Yuan, 37 N. Gupta and E. T. Farinas, Protein Eng., Des. Sel., 2010, 23,
X. C. Zhang and Y. Z. Xiao, PLoS One, 2014, 9(7), e102423.
679–682.
11 Z. Y. Zhao, J. Liu, M. Hahn, S. Z. Qiao, A. P. J. Middelberg and 38 M. Gunne and V. B. Urlacher, PLoS One, 2012, 7, e52360.
L. Z. He, RSC Adv., 2013, 3, 22008–22013.
12 L. O. Martins, C. M. Soares, M. M. Pereira, M. Teixeira,
39 S. Callejon, R. Sendra, S. Ferrer and I. Pardo, Appl. Microbiol.
Biotechnol., 2015, DOI: 10.1007/s00253-015-7158-0.
T. Costa, G. H. Jones and A. O. Henriques, J. Biol. Chem., 40 A. Marjasvaara, J. Janis and P. Vainiotalo, J. Mass Spectrom.,
2002, 277, 18849–18859.
2008, 43, 470–477.
13 K. Miyazaki, Extremophiles, 2005, 9, 415–425.
14 S. Brander, J. D. Mikkelsen and K. P. Kepp, J. Mol. Catal. B:
Enzym., 2015, 112, 59–65.
15 J. Ihssen, R. Reiss, R. Luchsinger, L. Thony-Meyer and
M. Richter, Sci. Rep., 2015, 5, 10465.
41 L. P. Christopher, B. Yao and Y. Ji, Frontiers in Energy
Research, 2014, 2(12), 1–13.
42 J. P. Kallio, S. Auer, J. Janis, M. Andberg, K. Kruus,
J. Rouvinen, A. Koivula and N. Hakulinen, J. Mol. Biol.,
2009, 392, 895–909.
16 L. Yin, Z. Shen, J. Niu, J. Chen and Y. Duan, Environ. Sci. 43 Y. Y. Wan, Y. M. Du and T. S. Miyakoshi, Sci. China, Ser. B:
Technol., 2010, 44, 9117–9122. Chem., 2008, 51, 669–676.
17 M. Z. Khan, P. K. Mondal and S. Sabir, J. Hazard. Mater., 44 G. Diamantidis, A. Effosse, P. Potier and R. Bally, Soil Biol.
2011, 190, 222–228.
Biochem., 2000, 32, 919–927.
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