Tetrahedron Asymmetry p. 4781 - 4790 (2000)
Update date:2022-08-24
Topics:
Baumann, Markus
Hauer, Bernhard H.
Bornscheuer, Uwe T.
Hydrolases showing high enantioselectivity towards three racemic alcohols (1-methoxy-2-propanol, 3-hydroxy-tetrahydrofuran, 3-butyn-2-ol) and pantolactone were identified by a step-wise screening procedure. Initially, those biocatalysts, which exhibited hydrolytic activity towards the corresponding acetates or butyrates, were selected out of >100 enzymes. Here, rapid screening was performed in a pH-indicator-based format in microtiter plates. Subsequently, enantioselectivity of active hydrolases was determined in small scale reactions (~1 mg substrate per reaction) by means of gas chromatography using chiral columns. Enzymes exhibiting highest enantioselectivities were then chosen for preparative scale resolution. Using this strategy, at least one suitable hydrolase was found for 3 out of the 4 model compounds examined, allowing efficient kinetic resolution. Moreover, in all cases enantiocomplementary enzymes were identified thus enabling access to both enantiomers of all substrates.
View MoreTIANJIN BAOLIRUITE CHEMICALS CO.,LTD
Contact:+86-022-58652605
Address:NO.60,xiqing high-end metal products industrial zone, Tianjin, China.
Yibin Hope Pharmaceutical Co., Ltd.
Contact:+86-831-5109851 86-831-5109836
Address:Luolong Industrial Zone,Nanxi, Yibin City, Sichuan, China. | Email: wishmarketing@gmail.com | Website: http://www.hopepharma.cc/
Wuxi Morality Chemical Co., Ltd
Contact:
Address:B/7F, 321th WuYun Rd, Wanda Plaza, Wuxi City, 214174, China
website:http://www.simagchem.com
Contact:+86-592-2680277
Address:21/F Hualong Office Building,No.6 Hubin East Road, Xiamen,China
Contact:86-21-34622192,13917187091,21-34622765
Address:No. 500 Caobao Road Shanghai P.R China
Doi:10.1134/S1070328417080036
(2017)Doi:10.1021/ja01315a096
(1935)Doi:10.1021/ja01044a044
(1969)Doi:10.1021/ic902490z
(2010)Doi:10.1246/cl.130188
(2013)Doi:10.1007/s10593-011-0785-1
(2011)