Chemistry of Heterocyclic Compounds 2019, 55(4/5), 455–462
the development of the virus-induced cytopathic effect on
12. Naumenko, K.; Zagorodnya, S.; Golovan, A.; Kovtyn, V.
the transplantable monolayer MDCK cell culture.15 The
MDCK cell culture was pre-grown for 24 h in 96-well
plates and washed twice from the growth medium with
Hanks solution. The test compounds were dissolved in
DMSO (final concentration 1 mg/ml), and then in a
DMEM supporting medium. Serial tenfold dilutions of the
virus-containing liquid in DMEM medium which contain
J. Adv. Biol. 2016, 9, 1896.
1
3. Pertseva, T. A.; Kireeva, T. V.; Cherkasova, А. V.; Bartus', E.
V. Ukrains'kii medichnyi chasopis 2012, (5), 67.
4. Cherkasov, A.; Muratov, E. N.; Fourches, D.; Varnek, A.;
Baskin, I. I.; Cronin, M.; Dearden, J.; Gramatica, P.;
Martin, Y. C.; Todeschini, R.; Consonni, V.; Kuz'min, V. E.;
Cramer, R.; Benigni, R.; Yang, C.; Rathman, J.; Terfloth, L.;
Gasteiger, J.; Richard, A.; Tropsha, A. J. Med. Chem. 2014,
57, 4977.
1
(
experiment) or do not contain (control) the test compound
were introduced in the wells with the monolayer of cell
culture. Evaluation of antiviral activity was carried out
after 48 h incubation in a thermostat at 37°C according to
the presence of virus-induced cytopathic action using a
Leica DM IL inverted microscope.
15. Preclinical Studies of Drugs: Guidelines [in Russian];
Stefanov, A. V.; Ed.; Avitsena: Kyiv, 2002.
1
1
6. Artemenko, A.; Muratov, E. J. Comp-Aid Mol Des. 2008, 22,
4
03.
7. Kuz'min, V.; Artemenko, A. G; Muratov, E. N.; Ognichenko, L. N.;
Hromov, A. I.; Liahovsky, A. V.; Polischuk, P. G. In National
Institute of Allergy and Infectious Dieseases, NIH;
Georgiev, V. St.; Western, K.; McGowan, J. J., Eds.; Humana
Press Inc.:Totowa, 2008, Vol. 1, p.163
The calculation of TCID50 for in vitro experiments was
carried out by the Kerber method in the modification of
Ashmarin according to the formula:
3
2
1
8. Kuz'min, V. E.; Artemenko, A. G.; Muratov, E. N.;
Polischuk, P. G.; Ognichenko, L. N.; Liahovsky, A. V.;
Hromov, A. I.; Varlamova, E. V. In Recent Advances in
QSAR Studies; Puzyn, T.; Leszczynski, J.; Cronin, M. T.,
Eds.; Springer: London, 2010, p. 127.
9. Polischuk, P.; Mokshina, E.; Kosinskaja, A.; Muats, A.;
Kulinsky, M.; Tinkov, O.; Ognichenko, L.; Khristova, T.;
Artemenko, A.; Kuz'min, V. In Advances in QSAR modeling:
logTCID50 = L – d(S – 0.5),
where L is the initial dilution in the experiment; d is the
difference between log of serial dilutions; S is the sum of
the proportions of the test objects that gave a positive result
1
(
agglutination or cytopathic effect).
The statistical significance of the results was determined
Applications
in
Pharmaceutical,
Chemical,
Food,
by the nonparametric character criterion for related
Agricultural and Environmental Sciences; Kunal, R., Ed.;
Springer: Cham, 2017, p. 107.
samples.40
2
0. Muratov, E. N.; Artemenko, A. G.; Varlamova, E. V.;
Polischuk, P. G.; Lozitsky, V. P.; Fedchuk, A. S.; Lozitska, R. L.;
Gridina, T. L.; Koroleva, L. S.; Sil'nikov, V. N.; Galabov, A. S.;
Makarov, V. A.; Riabova, O. B.; Wutzler, P.; Schmidtke, M.;
Kuz'min, V. E. Future Med. Chem. 2010, 2, 1205.
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The work was carried out with partial financial support
from the Ukrainian Science and Technology Center as part
of the partner project No. P407 (USA, GIPP) and project
No. 6385.
2
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