BALAKRISHNAN ET AL.
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oxidize 3,5‐DTBC to 3,5‐DTBQ in aerobic condition.
ESI‐MS studies were performed in the presence of 3,5‐
DTBC, explaining the possible complex–substrate intermedi-
ates. The X‐band EPR spectral results indicate that the metal
centres are involved in the catecholase activity. Ligand‐
centred radical generation was further confirmed by DFT cal-
culation. The phosphatase (4‐NPP) activity of the complexes
was investigated and the results demonstrate that the com-
pounds exhibit excellent activity in acetonitrile medium.
The interactions of the complexes with CT‐DNA and BSA
protein were investigated using absorption and fluorescence
titration methods. The studies reveal that all the complexes
strongly interact with CT‐DNA and BSA protein. The com-
plexes exhibit significant hydrolytic cleavage of supercoiled
pUC19 DNA. In vitro cytotoxicity results for the complexes
towards MCF7 and MIA‐PA‐CA‐2 cell lines demonstrate
that they exhibit significant cytotoxic activity. The molecular
docking technique was employed to determine the binding
affinity of the complexes with DNA and protein molecules.
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ACKNOWLEDGEMENTS
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The financial support received from the Science and
Engineering Research Board (SERB), Department of Science
and Technology (DST), Government of India, New Delhi
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10] a) C. Balakrishnan, S. Natarajan, M. A. Neelakantan, RSC Adv.
2016, 6, 102482; b) L. Subha, C. Balakrishnan, S. Thalamuthu,
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(
EMR‐II/2014/000081) and Board of Research in Nuclear
Sciences (BRNS), DAE‐BARC, Mumbai, India (no. 35/14/
3/2014) is gratefully acknowledged. C.B. is grateful to
0
CSIR, India for his fellowship. STIC, Cochin is acknowl-
edged for performing the NMR analysis.
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Sundaramoorthy, D. Velmurugan, Spectrochim. Acta A 2012, 91,
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