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RSC Advances
Page 7 of 9
DOI: 10.1039/C6RA08677B
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ARTICLE
Furthermore, many dark areas were surrounded by the areas
with strong fluorescent signal. The dark areas should be the
location of cell nucleus. Due to the absent of targeting agents
on the surface of PEGMAꢀIAꢀβꢀCD/AdꢀPhNH2 FPNs, the
internalization of PEGMAꢀIAꢀβꢀCD/AdꢀPhNH2 FPNs is mainly
ascribed to the nonspecific endocytosis, which has been
demonstrated by Liu et al.65 It is well known that the cell
nucleus pore is less than 30 nm, which is much smaller than the
size of PEGMAꢀIAꢀβꢀCD/AdꢀPhNH2 FPNs. Therefore, we
think that PEGMAꢀIAꢀβꢀCD/AdꢀPhNH2 FPNs are difficult to
enter cell nucleus directly and mainly distributed in the
cytoplasm. Considered the properties included their high water
dispersibility, low toxicity and desirable AIE active optical
properties, PEGMAꢀIAꢀβꢀCD/AdꢀPhNH2 FPNs are expected to
be promising candidates for biomedical applications.
Acknowledgements
This research was supported by the National Science
Foundation of China (Nos. 51363016, 21474057, 21564006,
21561022), and the National 973 Project (Nos.
2011CB935700).
Notes and references
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Fig. 7 CLSM images of cells incubated with 40 μg mL−1 of FPN for 3 h. A) Bright field, B)
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4. Conclusions
In summary, FPNs based on AIE dye and supermolecular
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radical polymerization, ringꢀopening reaction and hostꢀguest
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had not only strong fluorescence, uniform size and high water
dispersibility, but also possessed excellent biocompatibility,
making them promising candidates for various biomedical
applications. Furthermore, Because of the existence of carboxyl
groups at the PEGMAꢀIA, multifunctional platform for the
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βꢀCD could also be utilized for encapsulation of some
anticancer agents (e. g. paclitaxel, curcumin) or fabrication of
supermolecular hydrogels through host guest interaction.
Furthermore, the obtained FPNs with red emission show
important for fabricating fluorescent probes because of their
long excitation wavelength, which largely reduce the injury for
cells. Therefore, PEGMAꢀIAꢀβꢀCD/AdꢀPhNH2 FPNs are
expected to have a profound impact on the biomedicine fields
for above described advantages. Finally, many other AIE active
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4
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mentioned, the AIE active fluorescent supermolecular
assemblies were believed to be promising candidates for
fabrication of many multifunctional biomaterials.
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