J Fluoresc
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Fig. 7 Viability of L-929 cells cultured in complete DMEM with 60 μM
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Cytotoxicity Assay
The ligand safety was assessed after 24 hours treatment to the
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concentrations as showed in Fig. 7. The non-toxic behavior of
ligand 4 and its ability to track the Ni2+ in living cells suggest its
possibility to use in biological system as nickel sensor.
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Conclusion
In summary, 2-(2-fluorobenzoyl)-N-(2-methoxyphenyl)
hydrazinecarbothioamide (4) has been introduced as nickel
ion sensor in mixed aqueous-organic media. The
photophysical properties of synthesized ligand 4 exhibited
the fluorescence emission signal at 488 nm and fluorescence
excitation signal at 388 nm upon nickel ligation which was
considered due to partial generation of quinoline like structure
in four by ligand-nickel chelation. Furthermore, for practical
applicability, bio imaging experiment was conducted using
L-929 cell lines. The ligand four showed no toxicity with
absolute cell permeability for the tested cell lines. These
results depicts that the newly synthesized ligand 4 can be used
as selective nickel sensing platform in mixed aqueous-organic
solution and inside living cells with considerable fluorescence
response in the visible region.
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Acknowledgments This research was supported by the Basic Science
Research Program through the National Research Foundation of Korea
(NRF) funded by the Ministry of Education, (No. 2011–0015056) and the
National Research Foundation of Korea (NRF) grant funded by the Korea
government (MSIP) (NRF-2010-0027963).