6
BAI ET AL.
according to protocols. After infection, the mice were kept
in the cage with free food and water.
donor Schiff base ligand 2-(((E)-2-(((E)-2-hydroxybenzy-
lidene)amino)benzylidene)amino)phenol. The structure of
the as-prepared complex 1 has been characterized by single-
crystal X-ray diffraction studies, which demonstrate that com-
plex 1 has a rod-like 1-D chain bridged by Dca; the
Co(L) units are bridged by dca ligands along the a-axis. Fur-
thermore, the nanoparticles of complex 1 were prepared
using a green grinding approach, which has good water dis-
persibility. The CVB3-infected mice model was constructed
first; then, the inhibitory effect of nano 1 on mice virus loads
was measured by RT-PCR assay. Next, the cardiac functions
of mice were also detected after nano 1 treatment. All the
results in this study suggested that the compound could
reduce the virus burdens and improve the cardiac functions;
it has an excellent protective effect on CVB3-infected mice.
Molecular docking results show that the chain structures of
nitrogen on the Co(II) could improve the stability of binding
interactions between ligand and receptor, while oxygen-
containing functional groups have a negative influence on
the binding interaction, which shows the protective activity
mechanism from the molecular level.
3.5 | CVB3 virus burden detection
The BALB/c male were affected by CVB3 virus, inducing
the production of myocarditis, and then, nano 1 was injected
for mice treatment. Three and seven days after CVB3 virus
infection and nano 1 treatment, the cardiac tissue was col-
lected for total RNA extraction, and the RT-PCR assay was
performed to detect the loading of CVB3 virus in accordance
with the producer's instructions.[17] In brief, the RNeasy
Mini RNA Isolation Kit (QIAGEN) was used to extract the
total RNA, and the concentration of RNA was quantified.
Then, the total RNA was reverse transcripted into cDNA
with a cDNA Synthesis Kit (Takara), and the RT-PCR was
performed using the SYBR Green PCR Master Mix kit
(Takara). The primers used in this experiment are as follows:
CVB3 Forward: 50-ATCAAGTTGCGTGCTGTG-30; CVB3
reverse: 50-TGCGAAATGAAAGGA. The relative expression
levels of CVB3 was calculated by the 2−ΔΔCt method.
ACKNOWLEDGMENTS
This work was supported by grants from Research Pro-
jects of Institutions of Higher Learning in Inner Mongolia
(NJZY17195) and Inner Mongolia Natural Science Foun-
dation [2017MS(LH)0824].
3.6 | Echocardiography evaluation
The mice were infected with CVB3 virus to establish the
myocarditis disease model; then, the mice were treated
with nano 1 at the dosage of 5 mg/kg for treatment. The
heart function indicators are LVFS and LVEF, which
were evaluated by an echocardiography assay (Vevo2100,
Visual Sonics, Canada) at the seventh day after CVB3
contagion and nano 1 treatment.[18]
ORCID
REFERENCES
[1] (a) C. Tschöpe, L. T. Cooper, G. Torre-Amione, S. Van Linthout,
Circ. Res. 2019, 124, 1568; (b) D. Zhang, H. Q. Zhang, S. Zhao,
Z. G. Li, S. X. Hou, Int. J. Electrochem. Sci. 2019, 14, 4659.
[2] Y. Liu, X. Huang, Y. Liu, D. Li, J. Zhang, L. Yang, Exp. Ther.
Med. 2019, 17, 4471.
[3] S. Guo, R. Chen, H. Li, T. Zhang, Y. Liu, Int. J. Softw. Eng.
Know. 2019, 29, 139.
[4] J. Beuy, V. Wiwanitkit, Anatol. J. Cardiol. 2019, 21, 293.
[5] T. H. Noh, O. S. Jung, Acc. Chem. Res. 2016, 49, 1835.
[6] R. Canton-Vitoria, C. Stangel, N. Tagmatarchis, ACS Appl.
Mater. Interfaces 2018, 10, 23476.
[7] X. Feng, Y. Q. Feng, N. Guo, Y. L. Sun, T. Zhang, L. F. Ma,
L. Y. Wang, Inorg. Chem. 2017, 56, 1713.
[8] X. Feng, R. F. Li, L. Y. Wang, S. W. Ng, G. Z. Qin, L. F. Ma,
CrstEngComm 2015, 17, 7878.
[9] X. Feng, Y. Q. Feng, L. Liu, L. Y. Wang, H. L. Song, S. W. Ng,
Dalton Trans. 2013, 42, 7741.
3.7 | Molecular docking
In order to iterate over the conformational space of Co com-
plex when interacting with active cites provider, the stochas-
tic geometry searching approach has been used to sample all
the possible binding interactions. To address such a compli-
cated task, a modified version of AutoDock Vina, Smina,
has been used. The initial configuration of Co(II) was taken
from the experimental crystal structure and used for molecu-
lar docking directly. A maximum number of 25 binding
modes has been used. Visualization has been carried out by
using an open-source version of PyMOL v2.3.0.
[10] M. T. Kaczmarek, M. Zabiszak, M. Nowak, R. Jastrzab, Coord.
Chem. Rev. 2018, 370, 42.
4 | CONCLUSIONS
ꢀ
[11] R. Bikas, N. Noshiranzadeh, L. Sieron, H. Hosseini-Monfared,
In summary, a dicyanamide-bridged coordination polymer
has been successfully prepared by using the bidentate NO
J. M. Barandiaran, T. Lis, J. Alonso, Inorg. Chem. Commun.
2016, 67, 85.