Article
J. Agric. Food Chem., Vol. 57, No. 18, 2009 8295
ACKNOWLEDGMENT
Table 2. Concentrations of Falcarinol, Falcarindiol, and Falcarindiol 3-Acetate
in EtOAc Extracts from the Carrot Cultivars cv. Bolero (Carrot Extract 1) and
cv. Purple Haze (Carrot Extract 2)
a
We thank A. K. Nielsen and K. B. Poulsen for their skilled
technical assistance.
carrot
extract
falcarinol
(μg/mL)
falcarindiol
(μg/mL)
falcarindiol 3-acetate
(μg/mL)
Note Added After ASAP Publication
1
2
281.1
645.7
596.0
152.3
195.8
107.1
The original ASAP publication of August 20, 2009, did not
reflect all galley proof corrections. These have been incorporated
in the publication of September 1, 2009.
a
Concentrations are given in stock solutions of which different concentrations
were prepared in cell culture medium.
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extract giving an approximate 20% decrease in proliferation
(
(
Figure 4A). In FHs 74 Int. cells, there was no difference between
the effects of the two extracts (Figure 4B), but the effect at a given
concentration was larger than that in Caco-2 cells. At the highest
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The more potent inhibitory activity of carrot extract 2 than of
carrot extract 1 in Caco-2 cells (Figure 4A) is in accordance with
the approximately 3 times higher concentration of falcarinol in
carrot extract 2, as falcarinol is a more potent inhibitor than
falcarindiol (Figure 2). Comparing the effects of the carrot
extracts with the effects of falcarinol or falcarindiol alone, we
showed that the mixture of polyacetylenes found in the extracts
was more potent in inhibiting cell proliferation. As an example,
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1
00 times in cell culture medium (Table 2) contained falcarinol,
falcarindiol, and falcarindiol 3-acetate in concentrations of 2.81,
.96, and 1.96 μg/mL, respectively, and caused a reduction in
cell proliferation to 36%. For comparison, a concentration of
.5 μg/mL of falcarinol decreased proliferation to 68%, while
(
5
2
falcarindiol caused no decrease in cell proliferation (Figures 2
and 4, and Table 2). Concentrations in falcarinol of 5 μg/mL or
falcarindiol of 10-20 μg/mL were required to obtain a decrease in
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(
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2
cell proliferation. In FHs 74 Int. cells, a similar synergistic effect
can be suggested as carrot extract 1 or 2 both caused a decrease in
cell proliferation to 30and 6% inconcentrations of0.5% and 1%,
respectively, of the extracts. To obtain such decreases in cell
proliferation of FHs 74 Int. cells by falcarinol alone, a concentra-
tion of 10 μg/mL would be necessary.
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anticancer effect previously observed for a carrot diet compared to
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present results therefore clearly suggest that other metabolites in
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acetylenes in carrots, thereby affecting their effectiveness in vivo.
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