ꢀꢀꢀꢁ
286ꢀ ꢀQ. Wan et al.: Two new glycosidal metabolites of endophytic fungus Penicillium sp. (NO.4)
vacuum to give 20 g residue. This was subjected to silica (HyClone) supplemented with 10 % fetal bovine serum
gel CC and eluted with light petroleum to acetone, then to (FBS) (HyClone). The cells were maintained in 5 % CO2
methanol. The subfractions were merged to six fractions at 37 °C. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-
from TLC results. Fraction A (1.5 g) was further subjected 2H-tetrazolium bromide (MTT, Sigma) colorimetric assay
to C-18 reverse phase silica gel CC with gradient elution was used to evaluate cell proliferation in the presence of
from 20 % methanol-H2O to 80 % methanol-H2O; fraction the different chemicals. The cells were seeded in 96-well
A was fractionated into five subfractions. Subfractions A-4 culture plates and treated with either vehicle or desired
and A-5 were purified by repeated silica gel CC, eluting concentrations of the chemicals for a further 24 h. After
with light petroleum–acetone (4:1) and light petroleum– treatment, the cells were incubated at 37 °C with MTT (1
acetone (3:1) to yield compounds 1 (8 mg), 2 (10 mg), 3 (15 μL per well, 5 mg mL−1) for 4 h; cell growth response to
mg), 4 (10 mg), 5 (20 mg) and 6 (50 mg).
the chemicals was determined by measuring the absorb-
ance at 570 nm with a plate reader. Three replicates were
used for each treatment. In the anticancer activity in vitro
experiment, mitomycin was used as positive control for
the anticancer assay.
4.4 8-O-β-d-glucopyranosyl-6-methyl-
1-carboxylate methyl ester xanthone (1)
Colorless crystals, M.p. 166–168 °C. – UV/Vis (CH3OH):
λmax (lg εmax)ꢀꢁ=ꢁꢀ205 nm (2.4), 254 nm (3.9), 287 nm (3.5). – 4.8 Antimicrobial assays
[α]D25ꢀꢁ=ꢁꢀ–85 (cꢀꢁ=ꢁꢀ0.06, CH3OH). – IR(film): υꢀꢁ=ꢁꢀ3430, 2956,
1
13
2925, 2880, 1721, 1634, 1528, 1460 cm−1. – H and C NMR Antimicrobial assays against plant-pathogenic Erwinia caro-
data (see Table 1). – HRMS ((+)-ESI): m/zꢀꢁ=ꢁꢀ463.1244 tovora sub sp. Carotovora and Sclerotiumrol fsii Sacc. were
+
(cacld. 463.1240 for C22H23O11, [M+H] ) and 485.1063 (cacld. carried out using the well-diffusion method. Amphotericin
+
485.1060 for C22H22O11Na, [M+Na] ).
B was used as positive control for the antifungal assay.
Acknowledgments: This work was financial supported by
the Natural Science Foundation of China (21002058 and
21272137).
4.5 4′-O-β-d-galactopyranosyl djalonensone
(2)
Colorless crystals, M.p. 156–157 °C. – UV/Vis (CH3OH): λmax
(lg εmax)ꢀꢁ=ꢁꢀ208 nm (2.3), 255 nm (4.1), 287 nm (3.8), 330 nm
(2.6). – [α]D25ꢀꢁ=ꢁꢀ–68 (cꢀꢁ=ꢁꢀ0.07, CH3OH). – IR(film): υꢀꢁ=ꢁꢀ3363,
2924, 1668 (Cꢁ=ꢁO), 1609, 1448, 1342 cm−1. – 1H and 13C NMR
data (see Table 1). – HRMS ((+)-ESI): m/z:ꢀꢁ=ꢁꢀ435.1293
References
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