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Analytical Chemistry
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enzymolysis time and fluorogenic reaction time for ALP
detection, the interfere study of different concentrations of
UC and DA, the influence of antibody or antibody-ALP
conjugates on the fluorescence of the as-resulted
fluorophore, comparison of performance of different ALP
and AFP sensors, analytical results for ALP and AFP in the
human serum samples. (PDF).
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AUTHOR INFORMATION
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Corresponding Author
Author Contributions
The manuscript was written through contributions of all authors.
Notes
The authors declare no competing financial interest.
(17) Zhao, J. H.; Wang. S.; Lu, S. S.; Sun, J.; Yang, X. R. A
Luminescent Europium-dipicolinic Acid Nanohybrid for the Rapid
and Selective Sensing of Pyrophosphate and Alkaline Phosphatase
activity. Nanoscale 2018, 10, 7163−7170.
ACKNOWLEDGMENT
This work was supported by the Education Key Projects of Henan
Provincial Department (19A150002), the Scientific Research
Foundation of Luoyang Institute of Science and Technology
(2017BZ16), the National Natural Science Foundation of China
(Nos. 21605139, 21435005), and the Youth Innovation Promotion
Association, CAS (No. 2018258).
(18) Liu, Y.; Schanze, K. S. Conjugated Polyelectrolyte-based
Real-time Fluorescence Assay for Alkaline Phosphatase with
Pyrophosphate as Substrate. Anal. Chem. 2008, 80, 8605−8612.
(19) Kang, E. B.; Choi, C. A.; Mazrad, Z. A. I.; Kim, S. H.; In, I.;
Park, S. Y. Fluorescence Assay for Alkaline Phosphatase based on
ATP Hydrolysis-triggered Dissociation of Cerium Coordination
Polymer Nanoparticles. Anal. Chem. 2017, 89, 13508−13517.
(20) Qian, Z. S.; Chai, L. J.; Huang, Y. Y.; Tang, C.; Shen, J. J.;
Chen, J. R.; Feng, H. A. A Real-time Fluorescent Assay for the
Detection of Alkaline Phosphatase Activity based on Carbon
Quantum Dots. Biosens. Bioelectron. 2015, 68, 675−680.
(21) Li, G. L.; Fu, H. L., Chen, X. J.; Gong, P. W.; Chen, G.; Xia,
T.; Wang, H.; You, J. M.; Wu, Y. N. Facile and Sensitive
Fluorescence Sensing of Alkaline Phosphatase activity with
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(23) Xianyu, Y. L.; Wang, Z.; Jiang, X. Y. A Plasmonic
Nanosensor for Immunoassay via Enzyme-Triggered Click Chemistry.
ACS Nano 2014, 8, 12741−12747.
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