632
Z. Zhang et al. / Carbohydrate Polymers 79 (2010) 628–632
Table 2
Bosch, L., Harbers, E., & Heidelberger, C. (1958). Studies on fluorinated
pyrimidines. V. Effects on nucleic acid metabolism in vitro. Cancer
Research, 18, 335–343.
Bounous, G., Pageau, R., & Regoli, P. (1978). Enhanced 5-fluorouracil mortality in
rats eating defined formula diets. International Journal of Clinical Pharmacology
and Biopharmacy, 16, 265–267.
Release kinetics of 5-FU from three conjugates in different medium.
Mediums
0.1 M HCl
Conjugates
Equations
n
r
LP-5FU
P-5FU1
P-5FU2
Mt/M = 31.84t0.1464
0.1464
0.4403
0.3089
0.9801
0.9879
0.9950
1
Mt/M = 11.83t0.4403
1
Gao, H., Gu, Y. Q., & Ping, Q. N. (2007). The implantable 5-fluorouracil-loaded poly
Mt /M1 = 5.65t0.3089
(L-lactic acid) fibers prepared by wet-spinning from suspension. Journal of
Controlled Release, 118, 325–332.
Phosphate buffer
0.01 M NaOH
LP-5FU
P-5FU1
P-5FU2
Mt/M1 = 17.34t0.2312
0.2312
0.2536
0.4063
0.9913
0.9871
0.9874
Mt/M1 = 11.63t0.2536
Gretz, M. R., McCandless, E. L., Aronson, J. M., & Sommerfeld, M. R. (1983). The
galactan sulphates of the conchocelis phases of Porphyra leucosticta and Bangia
atropurpurea. Journal of Experimental Botany, 34, 705–711.
Hiller, S. A., Zhuk, R. A., & Lidak, M. Y. (1967). Pyrimidine nucleoside analogues. I.
N1-(a-furanidyl)derivatives of natural pyrimidine bases and their
antimetabolites. Doklady Akademii Nauk SSSR, 176, 332–335.
0.4063
Mt/M1 = 10.50t
LP-5FU
P-5FU1
P-5FU2
Mt/M = 45.66t0.1649
0.1649
0.4246
0.4421
0.9919
0.9890
0.9937
1
Mt /M = 9.75t0.4246
1
Mt/M1 = 13.53t0.4421
Jackson, T. G.,
&
Shirley, D. A. (1967). Friedel–Crafts acetylation and
chloroacetylation of the benzophenothiazines. The Journal of Organic
Chemistry, 32, 1190–1194.
Morgenstern, B.,
& Kammer, H. W. (1996). Solvation in cellulose–LiCl–DMAc
mechanism of the conjugate is a typical Fickian diffusion and con-
sistent with those result of experiments.
solutions. Trends in Polymer Science, 4, 87–92.
Myers, C. E. (1981). The pharmacology of the fluoropyrimidines. Pharmacological
Reviews, 33, 1–15.
Nishide, E., Ohno, M., Anzai, H., & Uchida, N. (1988). Extraction of porphyran from
Porphyra yezoensis Ueda F. Narawaensis Miura. Nippon Suisan Gakkaishi, 54,
2189–2194.
4. Conclusion
Notari, R. E., & Munson, J. W. (1969). Hydroxamic acids. I. Factors affecting the
stability of the hydroxamic acid–iron complex. Journal of Pharmaceutical
Sciences, 58, 1060–1064.
In this studies, we prepared three conjugates linking porphyran
with 5-FU and investigated their release mechanisms. The contents
of 5-FU loading were different due to the different reactive condi-
tion. The drug release durations of these conjugates were extre-
mely correlated with the concentration of drug loading, the pH of
media and molecular weight of the conjugates. The results repre-
sented that the release mechanism of all the conjugates was a typ-
ical Fickian diffusion. However, further in vivo studies on animal
models are necessary to establish the efficiency of the system.
Ouchi, T., Yuyama, H.,
& Vogl, O. (1985). Synthesis of poly(ethylene glycol)-
bound 3-(5-fluorouracil-1-yl)propanoic acid, its hydrolysis reactivity and
antitumor activity. Die Makromolekulare Chemie. Rapid Communications, 6,
815–819.
Ouchi, T., Banba, T., Fujimoto, M., & Hamamoto, S. (1989). Synthesis and antitumor
activity of chitosan carrying 5-fluorouracils. Macromolecular Chemistry and
Physics, 190, 1817–1822.
Ouchi, T., Banba, T., Matsumoto, S., & Suzuki, M. (1989). Antitumor activity of
chitosan and chitin immobilized 5-fluorouracils through hexamethylene
spacers via carbamoyl bonds. Journal of Bioactive and Compatible Polymers, 4,
362–371.
Philip, L. R.,
& Nicolas, A. P. (1987). A simple equation for description of
Acknowledgements
solute release. I. Fickian and non-Fickian release from non-swellable
devices in the form of slabs, spheres, cylinders or discs. Journal of
Controlled Release, 5, 23–36.
This work was supported by the Department of Science and
Technology of Shandong Province (2007BS07004). We are grateful
to Prof. Zuhong Xu and Prof. Zhien Li for their help in this
experiment.
Sirica, A. E., & Woodman, R. J. (1971). Selective aggregation of L1210 leukaemia cells
by the polycation chitosan. Journal of the National Cancer Institute, 47, 377–381.
Steele, J. W., & Anderson, W. K. (1972). Chloroacetylation of 1-azaphenothiazine.
Canadian Journal of Chemistry, 50, 1026–1029.
Suzuki, K., Tokoro, A., Owaka, Y., Suzuki, S., & Suzuk, M. (1986). Effect of N-acetyl
chitooligosaccharides on activation of phagocytes. Microbiology and
Immunology, 30, 777–787.
Zhang, X. F., Li, Y. X., Chen, X. S., Wang, X. H., Xu, X. Y., & Jing, X. B. (2005). Synthesis
and characterization of the paclitaxel/MPEG-PLA block copolymer conjugate.
Biomaterials, 26, 2121–2128.
Zhao, T. T., Zhang, Q. B., Qi, H. M., & Zhang, H. (2006). Degradation of porphyran
from Porphyra haitanensis and the antioxidant activities of the degraded
porphyrans with different molecular weight. International Journal of Biological
Macromolecules, 38, 45–50.
References
Aridoss, G., Balasubramanian, S., Parthiban, P., Ramachandran, R., & Kabilan, S.
(2007). Synthesis and antimicrobial activities of N-chloroacetyl-2,6-
diarylpiperidin-4-ones. Medicinal Chemistry Research, 16, 188–204.
Bohn, J. A., & BeMiller, J. N. (1995). (1 ? 3)-b-D-Glucans as biological response
modifiers: a review of structure–functional activity relationships. Carbohydrate
Polymers, 28, 3–14.