Bulletin of the Chemical Society of Japan p. 2501 - 2504 (1992)
Update date:2022-08-16
Topics:
Katayama, Atsushi
Kamidate, Tamio
Watanabe, Hiroto
Delayed chemilumunescence (CL) was observed in the copper(II)-catalyzed oxidation of cysteamine with oxygen in the presence of horseradish peroxidase (HRP) and luminol.After preferential catalytic oxidation of cysteamine by both Cu(II) and HRP, a HRP-catalyzed luminol CL reaction was subsequently commenced with hydrogen peroxide accumulated from the catalytic oxidation.Thus, a delay time from the reaction initiation to a sharp flash of CL was observed.The HRP-catalyzed CL-delay of luminol was applied to the determination of Cu(II).The delay time was linearly correlated with the Cu(II) concentration over the range from the detection limit of 5.0*10-9 to 1.5*10-5 M.The detection limit of the present method is a factor of 30 times better than that of the conventional luminol CL method.The relative standard deviation of the delay time in five succesive experiments was 3.2percent at 1.0*10-7 M of Cu(II).The present method was highly sensitive compared to the conventional luminol CL method.
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