Enzymatic reactions in Micrometer-Sized Compartments
J. Phys. Chem. B, Vol. 109, No. 4, 2005 1617
(13) Karlsson, M.; Sott, K.; Cans, A.; Karlsson, A.; Karlsson, R.; Orwar,
O. Langmuir 2001, 17, 6754-6758.
the number of enzyme molecules without performing additional
experiments.
(14) Karlsson, A.; Karlsson, M.; Karlsson, R.; Sott, K.; Lundqvist, A.;
Tokarz, M.; Orwar, O. Anal. Chem. 2003, 75, 2529-2537.
(15) Karlsson, M.; Davidson, M.; Karlsson, R.; Karlsson, A.; Bergen-
holtz, J.; Konkoli, Z.; Jesorka, A.; Lobovkina, T.; Hurtig, J.; Voinova, M.;
Orwar, O. Annu. ReV. Phys. Chem. 2004, 55, 613-649.
(16) Lundqvist, J. A.; Sahlin, F.; Aberg, M. A.; Stromberg, A.; Eriksson,
P. S.; Orwar, O. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 10356-10360.
(17) Kulin, S.; Kishore, R.; Helmerson, K.; Locascio, L. Langmuir 2003,
19, 8206-8210.
The technique presented here provides a tool that can be
applied to studies of confined chemical reactions, for example,
in developing biomimetic model systems for intracellular
biochemical reactions, or for investigating a few or even single
enzyme turnovers in a controlled environment. The results
presented in this paper stimulate further experimental investiga-
tions and theoretical modeling in spatially highly confined
environments, such as lipid nanotubes, where current theory
predicts a breakdown of classical kinetics.
(18) Karlsson, R.; Karlsson, M.; Karlsson, A.; Cans, A.; Bergenholtz,
J.; Akerman, B.; Ewing, A.; Voinova, M.; Orwar, O. Langmuir 2002, 18,
4186-4190.
(19) Dagdug, L.; Berezhkovskii, A. M.; Shvartsman, S. Y.; Weiss, G.
H. J. Chem. Phys. 2003, 119, 12473-12478.
(20) Huang, Z.; Olson, N. A.; You, W.; Haugland, R. P. J. Immunol.
Methods 1992, 149, 261-266.
(21) Hurtig, J.; Karlsson, M.; Orwar, O. Langmuir 2004, 20, 5637-
5641.
(22) Karlsson, M.; Nolkrantz, K.; Davidson, M. J.; Stromberg, A.;
Ryttsen, F.; Akerman, B.; Orwar, O. Anal. Chem 2000, 72, 5857-5862.
(23) Sott, K.; Karlsson, M.; Pihl, J.; Hurtig, J.; Lobovkina, T.; Orwar,
O. Langmuir 2003, 19, 3904-3910.
Acknowledgment. This work was supported by the Royal
Swedish Academy of Sciences through a donation by the
Wallenberg foundation, the Swedish Research Council (VR),
and the Swedish Foundation for Strategic Research (SSF).
Thanks to Dr. Mattias Karlsson and Daniel Fagerlund for help
with pictures.
References and Notes
(24) Helfrich, W. Z. Naturforsch. C 1973, 28, 693-703.
(25) Deuling, H. J.; Helfrich, W. Biophys. J. 1976, 16, 861-868.
(26) Karlsson, M.; Sott, K.; Davidson, M.; Cans, A. S.; Linderholm,
P.; Chiu, D.; Orwar, O. Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 11573-
11578.
(27) Derenyi, I.; Julicher, F.; Prost, J. Phys. ReV. Lett. 2002, 88,
238101.
(28) Evans, E.; Bowman, H.; Leung, A.; Needham, D.; Tirrell, D.
Science 1996, 273, 933-935.
(29) Karlsson, R.; Karlsson, A.; Orwar, O. J. Am. Chem. Soc. 2003,
125, 8442-8443.
(30) Chizmadzhev, Y. A.; Kuzmin, P. I.; Kumenko, D. A.; Zimmerberg,
J.; Cohen, F. S. Biophys. J. 2000, 78, 2241-2256.
(31) Stange, P.; Zanette, D.; Mikhailov, A.; Hess, B. Biophys. Chem.
1999, 79, 233-247.
(32) Leach, A. M.; Wheeler, A. R.; Zare, R. N. Anal. Chem. 2003, 75,
967-972.
(33) Lenghaus, K.; Dale, J.; Henderson, J.; Henry, D.; Loghin, E.;
Hickman, J. Langmuir 2003, 19, 5971-5974.
(1) Khairutdinov, R. F.; Serpone, N. Prog. React. Kinet. 1996, 21,
1-68.
(2) Konkoli, Z.; Karlsson, A.; Orwar, O. J. Phys. Chem. B 2003, 107,
14077-14086.
(3) Ismagilov, R. F.; Rosmarin, T. D.; Kenis, J. A.; Chiu, D. T.; Zhang,
W.; Stone, H. A.; Whitesides, G. M. Anal. Chem. 2001, 73, 4682-4687.
(4) Sia, S. K.; Whitesides, G. M. Electrophoresis 2003, 24, 3563-
3576.
(5) Kenis, P. J.; Ismagilov, R. F.; Whitesides, G. M. Science 1999,
285, 83-85.
(6) Tas, N.; Berenschot, J.; Mela, P.; Jansen, H.; Elwenspoek, M.; van
den Berg, A. Nano Lett. 2002, 2, 1031-1032.
(7) Haneveld, J.; Jansen, H.; Berenschot, E.; Tas, N.; Elwenspoek, M.
J. Micromech. Microeng. 2003, 13, S62-S66.
(8) Bolinger, P. Y.; Stamou, D.; Vogel, H. J. Am. Chem. Soc. 2004,
126, 8594-8595.
(9) Chiu, D.; Wilson, C.; Ryttsen, F.; Stromberg, A.; Farre, C.;
Karlsson, A.; Nordholm, S.; Gaggar, A.; Modi, B.; Moscho, A.; Garza-
Lopez, R.; Orwar, O.; Zare, R. Science 1999, 283, 1892-1895.
(10) Chiu, D. T.; Wilson, C. F.; Karlsson, A.; Danielsson, A.; Lundqvist,
A.; Stro¨mberg, A.; Ryttse´n, F.; Davidson, M.; Nordholm, S.; Orwar, O.;
Zare, R. N. Chem. Phys. 1999, 247, 133-139.
(34) Gribbon, P.; Hardingham, T. E. Biophys. J. 1998, 75, 1032-1039.
(35) Coleman, J. E. Annu. ReV. Biophys. Biomol. Struct. 1992, 21, 441-
483.
(36) Gray, J. Curr. Opin. Struct. Biol. 2004, 14, 110-115.
(37) Lu, H. P.; Xun, L.; Xie, X. S. Science 1998, 282, 1877-1882.
(11) Walde, P.; Ichikawa, S. Biomol. Eng. 2001, 18, 143-177.
(12) Karlsson, A.; Karlsson, R.; Karlsson, M.; Cans, A. S.; Stromberg,
A.; Ryttsen, F.; Orwar, O. Nature 2001, 409, 150-152.