Helvetica Chimica Acta p. 640 - 652 (1997)
Update date:2022-08-11
Topics:
Meggers, Eric
Kusch, Dirk
Giese, Bernd
The synthesis of a novel tris(bidentate ligand)ruthenium(II) complex 7 and its efficient tethering to the 5'-end of oligonucleotides is described. The resulting Δ- and Λ-isomeric ruthenium(II)-labelled oligonucleotides 10a-c and 11a-d were separated either by reversed-phase HPLC or by polyacrylamide gel electrophoresis. The diastereoisomerically pure isomers were fully characterized by UV/VIS and CD spectroscopy, mass spectrometry, and enzymatic digestion with base analysis. We also investigated the thermal denaturation of the hybridized double strands.
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