Bioorganic Chemistry p. 45 - 52 (2005)
Update date:2022-08-17
Topics:
Sievers, Annette
Wolfenden, Richard
The second order rate constant (kcat/Km) for decarboxylation of orotidine by yeast OMP decarboxylase (ODCase), measured by trapping 14CO2 released during the reaction, is 2 × 10-4 M-1 s-1. This very low activity may be compared with a value of 3 × 107 M-1 s-1 for the action of yeast OMP decarboxylase on the normal substrate OMP. Both activities are strongly inhibited by 6-hydroxy UMP (BMP), and abrogated by mutation of Asp-96 to alanine. These results, in conjunction with the binding affinity of inorganic phosphate as a competitive inhibitor (Ki = 7 × 10-4 M), imply an effective concentration of 1.1 × 109 M for the substrate phosphoryl group in stabilizing the transition state for enzymatic decarboxylation of OMP. The observed difference in rate (1.5 × 1011-fold) is the largest effect of a simple substituent that appears to have been reported for an enzyme reaction.
View MoreYantai Derun Liquid Crystal Materials Co. Ltd.
website:http://www.ytderun.com
Contact:86-535-6300169
Address:ROOM 90, XIANGFU STREET, FUSHAN NEW-HIGH-TECH IDUSTRY ZONE, YANTAI
Contact:+(852) 301-98033
Address:Flat C, 23/F, Lucky Plaza, 315-321 Lockhart Road, Wan Chai, Hong Kong
Beijing Century Richap Chemistry Co.,Ltd
Contact:+86- 010-64455497
Address:Guannan County, Lianyungang City, Jiangsu Province, China
Suzhou Sinosun Imp.&Exp. Corporation
website:http://www.szsinosun.com
Contact:+86-512-63488895,63488616
Address:No.758 East Jiangling Road Wujiang Economic & Technological Developmenty Zone Jiangsu China
Xinchang Jiu Xin Pharmaceutical Co., Ltd
website:http://www.jiuxinpharm.com
Contact:86 137 5756 8585
Address:Poyang industry Park
Doi:10.1002/(SICI)1099-1395(199609)9:9<639::AID-POC833>3.0.CO;2-Q
(1996)Doi:10.1039/b419088b
(2005)Doi:10.1016/j.catcom.2015.02.009
(2015)Doi:10.1021/jp953044g
(1996)Doi:10.1016/j.tetlet.2017.06.027
(2017)Doi:10.1039/jr9400000683
(1940)