Canadian Journal of Chemistry p. 383 - 389 (1990)
Update date:2022-08-22
Topics:
Singh
Roy
Preparation and properties of the bacteriorhodopsin (bR) analogue having the 3,7-dimethyl-9-(9-anthryl)-2E, 4E,6E,8E-nonatetraenal (12) chromophore is described. Synthesis of the chromophore has been achieved by successive introduction of C5 units to 9-anthraldehyde (3) via the Horner reaction. The all-trans chromophore has been characterized by its ultraviolet-visible and 1H nuclear magnetic resonance spectra. Incubation of 12 with bacterioopsin suspension (prepared by photobleaching of bR isolated from Halobacterium halobium) at ambient temperature in the dark gave the new bR analogue 15, which showed an absorption band at 545 nm, and an opsin shift of 5575 cm-1. The new pigment is stable to hydroxyl amine in the dark. It showed light-dark adaptation with the light-adapted from absorbing at a slightly red-shifted value of 550 nm. All-trans-retinal did not replace the anthryl chromophore in competitive bindings. Photolysis of the bR analogue 15, followed by difference spectrophotometric analysis, indicated formation of a photoproduct with an absorption band near 400 nm. The results are discussed in terms of the steroelectronic requirements of the bR reaction centre.
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