18
S. Spisani et al. / European Journal of Pharmacology 469 (2003) 13–19
À 5
gene cluster in human phagocytes. Biochem. Biophys. Res. Commun.
01, 174–179.
peak (10
higher than that of fMLP-OMe.
M) corresponds to a concentration 10 times
2
Fabbri, E., Spisani, S., Biondi, C., Barbin, L., Colamussi, M.L., Cariani, A.,
Traniello, S., Torrini, I., Ferretti, M.E., 1997. Two For-Met-Leu-
Phe.OMe analogues trigger selective neutrophil responses. A differential
As a general consideration, 3 proved to be the strongest
hydrophilic full agonist in all biological activities, while 2
was demonstrated to be the weakest (partial) agonist among
the test derivatives: these data have been well confirmed by
competition binding experiments.
2
+
effect on cytosolic free Ca . Biochim. Biophys. Acta 1359, 233–240.
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activate chemotaxis and respiratory burst selectively as tools for study-
ing human neutrophil responses. Cell. Signal. 6, 91–101.
From these data emerges a new picture of the receptor
pocket in which the third residue is accommodated: it can
accept even hydrophilic residues and is indifferent to residue
steric hindrance.
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S., Spisani, S., 2001. Modulation of neutrophil phospholipase C activ-
ity and cyclic AMP levels by fMLP-OMe analogs. Cell. Signal. 13,
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Acknowledgements
This work was supported by the Ministero dell’Univer-
sit a` e della Ricerca Scientifica e Tecnologica (MURST:
research funds 40% and 60%). We are grateful to Banca del
Sangue of Ferrara for providing fresh blood, and Dr. Selena
Harrison, from King’s College London, for the English
revision of the text.
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