anaerobic metabolism of Fe(III)-reducing bacteria provides
the electrons needed for reductive dechlorination.
Reaction Products. Between 80 and 90% of the CT that
was transformed by microbially reduced goethite and abiotic
suspensions of goethite with sorbed Fe(II) accumulated as
CF. No methylene chloride or chloromethane were found
during the headspace analysis. Several possible pathways
have been presented that may account for the remainder of
the reaction products (8, 9, 41). The primary electron-transfer
reaction yields an intermediate trichloromethane radical.
Further reduction of this radical can result in the formation
of dichlorocarbene that upon hydrolysis converts to either
CO or formate. We think it likely, therefore, that either formate
or CO comprise the other reaction products in the sorbed-
Fe(II)/ goethite system.
(15) Kostka, J. E.; Nealson, K. H.; Stucki, J. W. Clays Clay Miner. 1996,
4, 522.
4
(
(
(
16) Heijman, C. G.; Holliger, C.; Glaus, M. A.; Schwarzenbach, R.
P.; Zeyer, J. Appl. Environ. Microbiol. 1993, 59, 4350.
17) Heijman, C. G.; Grieder, E.; Holliger, C.; Schwarzenbach, R. P.
Environ. Sci. Technol. 1995, 29, 751.
18) Klausen, J.; Tr o¨ ber, S. P.; Haderlein, S. B.; Schwarzenbach, R.
P. Environ. Sci. Technol. 1995, 29, 2396.
(19) Rossello-Mora, R.; Caccavo, Jr., F.; Springer, N.; Spring, S.;
Osterlehner, K.; Shuler, W.; Ludwig, R.; Amann, R.; Schleifer, K.
H. Syst. Appl. Microbiol. 1994, 18, 569.
(
20) Truex, M. J.; Peyton, B. M.; Valentine, N. B.; Gorby, Y. A.
Biotechnol. Bioengin. 1997, 55, 490.
(
(
(
(
21) Goodman, B. A.; Lewis, D. G. J. Soil Sci. 1981, 32, 351.
22) Cihacek, L. J.; Bremner, Soil. Sci. Soc. Am. J. 1979, 43, 821.
23) Stookey, L. L. Anal. Chem. 1970, 42, 779.
24) Lovley, D. R.; Phillips, E. J. P. Appl. Environ. Microbiol. 1988,
5
4, 1472.
Acknowledgments
(25) Hering, J. G.; Stumm, W. Oxidative and Reductive Dissolution
of Minerals. In Reviews in Mineralogy; Ribbe, P. H., Ed.;
Mineralogical Society of America: Washington, 1990; pp 427-
We thank Jim Fredrickson for helpful suggestions. Pacific
Northwest National Laboratory is operated by Battelle
Memorial Institute for the U.S. Department of Energy under
contract DE-AC06-76RL01830. This work was funded in part
by the Office of Science and Technology, within the Depart-
ment of Energy’s Office of Environmental Management,
under the SC Focus Area.
4
65.
(
26) Stumm, W. Redox Processes Mediated by Surfaces. In Chemistry
of the Solid-Water Interface; Stumm, W., Ed.; John Wiley and
Sons: New York, 1992; pp 309-335.
(27) Honeyman, B. D.; Santschi, P. H. Environ. Sci. Technol. 1988,
22, 862.
(
(
(
28) Rustad, J. R.; Felmy, A. R.; Hay, B. P. Geochim. Cosmochim. Acta
996, 60, 1563.
1
Literature Cited
29) Yates, D. E.; Grieser, F.; Cooper, R.; Healy, T. W. Australian J.
Chem. 1977, 30, 1655.
30) Hayes, K. F. Equilibrium, Spectroscopy, and Kinetic Studies of
Ion Adsorption at the Oxide/Aqueous Interface; 1987; Stanford,
CA.
31) Dzombak, D. A.; Morel, F. M. M. Surface Complexation Modeling;
Hydrous Ferric Oxide; John Wiley & Sons: New York, 1990.
32) Sposito, G. The Surface Chemistry of Soils; Oxford University
Press: New York, 1984.
33) Stumm, W.; Morgan, J. J. Aquatic Chemistry: An Introduction
Emphasizing Chemical Equilibria in Natural Waters; John Wiley
(
(
(
(
1) Ramanand, K.; Balba, M. T.; Duffy, J. Appl. Environ. Microbiol.
993, 59, 3266.
2) Wilson, B. H.; Smith, G. B.; Rees, J. F. Environ. Sci. Technol.
986, 20, 997.
3) Brown, J. F.; Bedard, D. L.; Brennan, M. J.; Carnahan, J. C.; Feng,
H.; Wagner, R. E. Science 1987, 236, 709.
4) Gorby, Y. A.; Amonette, J. E.; Fruchter, J. S. Remediation of
Subsurface Materials by a Metal-Reducing Bacterium. In
Proceedings of the 33rd Annual Hanford Life Sciences Symposium
on Health and the Environment; San Diego, CA, Battelle Press:
1
1
(
(
(
1
994.
&
Sons: New York, 1996.
(
(
5) Semprini, L.; Hopkins, G. D.; McCarty, P. L.; Roberts, P. V.
Environ. Sci. Technol. 1992, 26, 2454.
(34) Baes, C. F.; Mesmer, R. E. The Hydrolysis of Cations; John Wiley
& Sons: New York, 1976.
(35) White, A. F.; Yee, A. Geochim. Cosmochim. Acta 1985, 49, 1263.
(36) Wehrli, B. Redox Reactions of Metal Ions at Mineral Surfaces.
In Aquatic Chemical Kinetics; Stumm, W., Ed.; John Wiley &
Sons: New York, 1990.
(37) Stumm, W.; Sulzberger, B. Geochim. Cosmochim. Acta 1992, 56,
3233.
38) Picardal, F. W.; Arnold, R. G.; Couch, H.; Little, A. M.; Smith, M.
E. Appl. Environ. Microbiol. 1993, 59, 3763.
39) Phelps, T. J.; Murphy, E. M.; Pfifner; S. M.; White, D. C. Microb.
Ecol. 1994, 28, 335.
40) Jones, J. G.; Simon, B. M. FEMS 1984, 21, 47.
41) Criddle, C. S.; McCarty, P. L. Environ. Sci. Technol. 1991, 25,
6) McCarty, P. L.; Rittman, B. E.; Bouwer, E. J. Microbiological
Processes Affecting Chemical Transformations in Groundwater.
In Groundwater Pollution Microbiology; Bitton, G., Gerba, C. P.,
Eds.; Wiley-Interscience: New York, 1984; pp 89-115.
7) Mohn, W. W.; Tiedje, J. M. Microbiol. Rev. 1992, 56, 482.
8) Kriegman-King, M. R.; Reinhard, M. Environ. Sci. Technol. 1992,
(
(
2
6, 2198.
9) Kriegman-King, M. R.; Reinhard, M. Environ. Sci. Technol. 1994,
8, 692.
(
(
2
(
10) Amonette, J. E.; Szecsody, J. E.; Schaef, H. T.; Templeton, J. C.;
Gorby, Y. A.; Fruchter, J. S. Abiotic Reduction of Aquifer Materials
by Dithionite: A Promising In Situ Remediation Technology. In
(
(
(
3
3rd Hanford Symposium on Health and the Environment; San
Diego, CA, Battelle Press: 1994.
9
73.
(
(
(
11) Lovley, D. R. Annu. Rev. Microbiol. 1993, 47, 263.
12) Lovley, D. R.; Reynolds, R. L. EOS. 1987, 68, 1258..
13) Roden, E. E., Zachara, J. M. Environ. Sci. Technol. 1996, 30,
Received for review December 8, 1999. Revised manuscript
received June 21, 2000. Accepted July 19, 2000.
1
618.
14) Kostka, J. E.; Nealson, K. H. Environ. Sci. Technol. 1995, 29,
535.
(
2
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