2
38
FLEKHTER et al.
was 0.39 g (90%); R 0.45; mp 135–138°ë (CHCl –
MeOH); H NMR: 0.79, 0.91, 0.95, 0.99, 1.08 (21 H,
ACKNOWLEDGMENTS
f
3
1
This work was supported by the Russian Foundation
for Basic Research (project no. 05-03-32832) and partly
6
s, 7ëç ), 1.10–1.80 (23 H, m, ëç , CH), 4.25 and
3
2
4
.45 (2 H, two d, J 11, H28), 4.75 (1 H, m, H3), 5.13 (1 supported by the UNESCO grant IBSP/4-VN-01.
H, s, H19), 7.25 and 7.35 (2 H, two dd, J 3.8, 4.7, H5'),
.20 and 8.28 (2 H, two ddd, J 4.7, 1.6, 1.7, H4'), 8.65
and 8.70 (2 H, two t, J 4.1, H6'), 9.14 and 9.18 (2 H, two
8
REFERENCES
1
3
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1
3
5
1
1
8.1, 21.0, 25.3, 26.2, 27.4, 28.0, 29.3, 30.7, 30.8, 31.4,
2.2, 32.7, 33.5, 37.2, 38.0, 38.2, 38.6, 38.9, 40.8, 43.2,
1.0, 55.6, 65.8 (C28), 82.3 (C3), 123.2, 123.2, 126.3,
26.7, 133.9 (C19), 136.9, 136.9, 137.8 (C18), 150.9,
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. Kurokawa, M., Basnet, P., Ohsugi, M., Hozumi, T.,
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H 8.34, N 4.04. C H N O (M 652.914). Calculated,
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2
56
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. Hostettmann-Kaldas, M. and Nakanishi, K., J. Med.
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. Sun, I.C., Kashiwada, Y., Morris-Natschke, S.L., and
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.5 ml MeOH was added, the solvent was evaporated in
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. Lee, K.-H., Chang, F.R., Sakurai, Y., and Chen, C.H.,
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2
0
0
.36 g (78%); R 0.46; mp 213–214°ë (EtOH), [α ]
+
D
and Kingston, D.G.I., Org. Lett., 2001, vol. 3, pp. 4047–
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4
049.
2
D
0
3
3° (c 1.00, CHCl ) (lit. mp 210–212°ë, [α ] + 29° [5];
3
10. Tolstikov, G.A., Flekhter, O.B., Shul’ts, E.E., Baltina, L.A.,
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2
D
0
1
mp 222°ë, [α ] + 29° [21]); H NMR: 0.78, 0.93,
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.97, 1.00, 1.02, 1.05, 1.08 (21 H, 7 s, 7ëç ), 1.10–2.20
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11. Krasutsky, P.A., Nat. Prod. Rep., 2006, vol. 23, pp. 919–
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2
13
1
1
2. Davy, G.S., Halsall, T.G., and Jones, E.R.H., Chem. Ind.,
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1
2
3
1
5.9, 26.7, 29.0, 29.2, 30.2, 31.9, 33.1, 33.3, 33.4, 33.6,
3.9, 36.8, 39.7, 40.4, 41.3, 42.4, 47.1, 47.8, 50.3, 54.7,
33.3 (C19), 136.4 (C18), 181.9 (C28), 218.3 (C3).
1
4. Davy, G.S., Halsall, T.G., Jones, E.R.H., and Meakins, G.D.,
Found, %: C 79.40, H 10.56. C H O (M 454.690).
3
0
46
3
r
J. Chem. Soc., 1951, pp. 2702–2705.
Calculated, %: C 79.25, H 10.20.
1
1
5. Vystrcil, A., Stejskalova-Vondraskova, E., and Cerny, J.,
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solved in 10% ethanol and then twofold successive
serial dilutions were prepared on the support medium
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churina, L.T., Galin, F.Z., Zarudii, F.S., Tolstikov, G.A.,
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1
7. Medvedeva, N.I., Flekhter, O.B., Kukovinets, O.S.,
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Rus. Akad. Nauk, Ser. Khim., 2007, no. 4, pp. 804–806.
1
99 (Sigma Chemical Co.). As a quantitative criterion 18. Flekhter, O.B., Baltina, L.A., Spirikhin, L.V., Baikova, I.P.,
and Tolstikov, G.A., Izv. Rus. Akad. Nauk, Ser. Khim., 1998,
of the observable antiviral action, a decrease in the
virus titer was determined in comparison with the con-
trol. The 50% effective concentrations (EC ) of the
no. 3, pp. 531–534.
1
2
9. Flekhter, O.B., Medvedeva, N.I., and Suponitsky, K.Yu.,
50
Acta Crystallogr., 2007, E63, pp. o2289–o2290.
researched substances under study were also calcu-
lated. The maximal tolerable concentrations of the
compounds were determined after the incubation with
a not infected cell culture for 72 h at 37°ë.
0. Flekhter, O.V., Karachurina, L.T., Nigmatullina, L.R.,
Sarozhnikova, T.A., Baltina, L.A., Zarudii, F.S.,
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sunova, O.A., and Pokrovskii, A.G., Rus. J. Bioorg.
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY Vol. 35 No. 2 2009