Journal of Pharmaceutical Sciences p. 280 - 283 (1988)
Update date:2022-08-30
Topics:
Iwaki
Ogiso
Ito
To understand the reaction between hydralazine (HP) or its acetone hydrazone (HAH), a metabolite of HP and pyruvic acid, a new selective HPLC method for simultaneous determination of HP, HAH, and hydralazine pyruvic acid hydrazone (HPH) was developed. In vitro degradation of HAH and formation HP and HPH were investigated at pH 7.4 and 37°C in the presence or absence of pyruvic acid. Hydralazine degraded slowly according to an apparent first-order rate (7.46 x 10-2 h-1). The degenerative reaction of HAH, accompanied by simultaneous hydrolysis to the parent drug HP, was also subject to apparent first-order loss (3.00 x 10-1 h-1). In addition, HAH was partly converted to HP and HPH in the presence of pyruvic acid. For the formation pathway of HPH, a model that included the direct reaction of HAH with pyruvic acid and the secondary formation mediated by back-conversion to HP gave a better fit to the experimental data than the model consisting of the latter reaction only. About 10% of the HPH formed was generated by the direct reaction of HAH with pyruvic acid, based on the rate constants estimated. These results suggest that the formation of HPH is not all accomplished through back-conversion to HP.
View MoreChemsigma International Co.,Ltd.
website:http://www.chemsigma.com
Contact:86-025-58748998
Address:Rm.705, 15th Building,Rd.Xinke II
He Bei Shun Er Chemical Co., LTD.
Contact:86-0311-86996932/86860168
Address:No 18,North street
shijiazhuang shuanglian chemical industry co.,ltd
Contact:0311-82190302
Address:Luquan Intersection , Shijiazhuang--Taiyuan Expressway,Shijiazhuang City
Taixing Joxin Bio-tec Co.,Ltd.
website:http://www.joxbio.com
Contact:86-523-87558858 87612088
Address:No.88, chengdong industrial park
ABA Chemicals (Shanghai) Limited
Contact:021- 5115 9199-232
Address:Suite 18D, #201 Ningxia Road,
Doi:10.1039/b905656d
(2009)Doi:10.1016/j.tet.2004.10.107
(2005)Doi:10.1016/S0040-4020(01)80420-8
(1993)Doi:10.1016/j.tetlet.2016.09.006
(2016)Doi:10.1002/chem.201700995
(2017)Doi:10.1002/ejic.200400092
(2004)