T. Morikawa et al. / Phytochemistry Letters 5 (2012) 53–58
57
Table 3
Effects of flavonoid constituents from S. sarmentosum on oleic acid–albumin-induced triglyceride accumulation in HepG2 cells.
TG/protein (% of control)
Conc. (
m
M)
0
0.1
1
10
59.3 ꢂ 0.6
100
52.0 ꢂ 1.0
b
b
b
b
b
b
b
Sarmenoside V (1)
100.0 ꢂ 2.0
100.0 ꢂ 1.7
100.0 ꢂ 2.6
100.0 ꢂ 3.3
100.0 ꢂ 2.3
100.0 ꢂ 2.1
100.0 ꢂ 5.2
100.0 ꢂ 2.0
100.0 ꢂ 4.1
100.0 ꢂ 4.6
100.0 ꢂ 3.8
100.0 ꢂ 4.9
100.0 ꢂ 2.0
100.0 ꢂ 2.2
100.0 ꢂ 1.4
100.0 ꢂ 7.5
100.0 ꢂ 1.2
100.0 ꢂ 8.1
100.0 ꢂ 3.3
100.0 ꢂ 2.7
100.0 ꢂ 2.4
100.0 ꢂ 3.2
100.0 ꢂ 1.1
100.0 ꢂ 0.6
100.0 ꢂ 1.1
71.6 ꢂ 2.0
66.1 ꢂ 2.0
Sarmenoside VI (2)
–
102.4 ꢂ 1.6
84.9 ꢂ 1.3
92.6 ꢂ 1.6
87.2 ꢂ 3.2
86.9 ꢂ 1.3
92.7 ꢂ 1.6
85.8 ꢂ 1.3
86.8 ꢂ 3.2
93.1 ꢂ 1.7
105.6 ꢂ 2.8
96.8 ꢂ 3.6
90.0 ꢂ 2.7
87.5 ꢂ 5.9
111.0 ꢂ 1.8
77.2 ꢂ 2.8
92.1 ꢂ 1.9
104.0 ꢂ 4.0
99.6 ꢂ 2.7
87.1 ꢂ 1.9
98.2 ꢂ 1.0
94.6 ꢂ 2.3
100.9 ꢂ 2.1
93.2 ꢂ 2.6
93.4 ꢂ 1.4
99.9 ꢂ 2.3
88.0 ꢂ 1.6
92.9 ꢂ 5.7
87.0 ꢂ 3.4
87.8 ꢂ 2.3
87.4 ꢂ 1.8
81.3 ꢂ 1.4
86.8 ꢂ 1.0
89.0 ꢂ 1.4
107.4 ꢂ 7.2
90.4 ꢂ 3.3
95.8 ꢂ 0.9
96.3 ꢂ 2.2
107.6 ꢂ 6.9
72.9 ꢂ 3.3
92.0 ꢂ 2.3
99.6 ꢂ 2.7
92.2 ꢂ 4.7
83.3 ꢂ 1.8
91.6 ꢂ 3.6
93.6 ꢂ 1.5
99.4 ꢂ 1.6
91.2 ꢂ 1.0
86.7 ꢂ 1.2
95.4 ꢂ 0.7
74.2 ꢂ 2.4
101.8 ꢂ 2.4
85.5 ꢂ 2.0
79.4 ꢂ 1.4
81.9 ꢂ 1.8
72.0 ꢂ 0.4
77.8 ꢂ 1.3
85.0 ꢂ 2.0
113.2 ꢂ 1.0
80.7 ꢂ 4.6
90.5 ꢂ 1.6
80.5 ꢂ 4.6
92.2 ꢂ 3.0
70.8 ꢂ 0.7
85.0 ꢂ 1.4
103.6 ꢂ 2.5
83.5 ꢂ 3.4
79.3 ꢂ 0.8
87.3 ꢂ 0.7
80.4 ꢂ 0.3
92.6 ꢂ 1.0
93.9 ꢂ 3.2
72.7 ꢂ 0.6
b
Sarmenoside VII (3)
87.4 ꢂ 1.8
–
Apigenin 7-O-Glc
b
b
b
b
b
b
b
b
b
b
b
b
b
b
Luteolin 7-O-Glc
96.4 ꢂ 2.4
91.3 ꢂ 2.7
95.8 ꢂ 3.4
85.4 ꢂ 1.1
87.5 ꢂ 1.1
108.2 ꢂ 1.4
–
Tricin 7-O-Glc
Kaempferol 7-O-Glc
b
b
b
b
Quercetin 7-O-Glc
Isorhamnetin 7-O-Glc
Tamarixetin 7-O-Glc
Kaempferol 3-O-Glc-(1 ! 2)-Rha-7-O-Rha
Sarmenoside I
b
b
b
104.9 ꢂ 2.7
98.9 ꢂ 1.6
92.2 ꢂ 2.4
–
b
b
Grosvenorine
Quercetin 3,7-di-O-Rha
Quercetin 3-O-Glc-(1 ! 2)-Rha-7-O-Rha
Sarmenoside II
b
b
a
b
a
b
b
81.5 ꢂ 3.0
98.9 ꢂ 1.8
–
Isorhamnetin 3-O-Glc-(1 ! 2)-Rha-7-O-Rha
Sarmenoside IV
b
b
b
b
Isorhamnetin 3-O-Glc-7-O-Rha
Isorhamnetin 3,7-di-O-Glc
102.2 ꢂ 3.0
103.1 ꢂ 2.1
95.6 ꢂ 5.4
95.1 ꢂ 3.1
–
b
b
Sinocrassoside C
1
Herbacetin 8-methyl ether 3,7-di-O-Glc
Limocitrin 3-O-Glc
b
b
Limocitrin 3,7-di-O-Glc
Bezafibrate
–
a
b
90.5 ꢂ 1.3
a
b
Each value represents the mean ꢂ S.E.M. (n = 4); Significantly different from the control, p < 0.05, p < 0.01.
(
1
60:40, v/v)] to furnish capitatin (1.2 mg, 74%) (Wang et al.,
984).
and Technology of Japan (MEXT), 2007–2011 and by a Grant-
in Aid for Scientific Research from MEXT. M. Y., H. M., and S. N.
were supported by the 21st COE Program, Academic Frontier
3
.6. Inhibitory effects on oleic acid–albumin-induced triglyceride
Project, and
MEXT.
a Grant-in Aid for Scientific Research from
accumulation in HepG2 cells
HepG2 cells (Dainippon Pharmaceuticals, Osaka, Japan) were
maintained in Minimum Essential Medium Eagle (MEM, Sigma–
Aldrich) containing 10% fetal bovine serum, 1% MEM non-essential
amino acids (Invitrogen), penicillin G (100 units/mL), and strepto-
Appendix A. Supplementary data
mycin (100
inoculated in 48-well tissue culture plate [10 cells/well in 200
well in MEM]. After 20 h, the medium was replaced with 200 L/well
2
mg/mL) at 37 8Cunder 5% CO atmosphere. The cells were
5
mL/
m
of Dulbecco’s modified Eagle’s medium (DMEM) containing low-
glucose (1000 mg/L), 5% (v/v) oleic acid–albumin (Sigma–Aldrich),
and a test sample. The cells were cultured for 4 d and replacement
with the fresh medium every 2 d. Then the medium was removed,
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Acknowledgements
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2
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T.M., K.N., and O.M. were supported by ‘High-Tech Research
Center’ Project for Private Universities: matching fund subsidy
from the Ministry of Education, Culture, Sports, Science
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