Central European Journal of Chemistry p. 1740 - 1748 (2013)
Update date:2022-08-28
Topics:
Dimcheva, Nina
Horozova, Elena
Ivanov, Yavor
Godjevargova, Tzonka
The immobilisation of AChE enzyme through chemisorption on Au-modified graphite was examined with view of its prospective application in the design of membraneless electrochemical biosensors for the assay of enzyme inhibitors. The developed immobilisation protocol has been based on a two-stage procedure, comprising i) electrodeposition of gold nanostructures on spectroscopic graphite; followed by ii) chemisorption of the enzyme onto gold nanoparticles. Both the coverage of the electrode surface with Au nanostructures and the conditions for enzyme immobilisation were optimised. The proposed electrode architecture together with the specific type of enzyme immobilisation allow for a long-term retaining of the enzyme catalytic activity. The extent of inhibition of the immobilised acetylcholinesterase enzyme by the organophosphorous compound monocrotophos has been found to depend linearly on its concentration over the range from 50 to 400 nmol mL-1 with sensitivity 77.2% inhibition per 1 μmol mL-1 of monocrotophos. [Figure not available: see fulltext.]
View MoreQingdao Kingway Pharmtech Co., Ltd.
Contact:86-532-87118899
Address:No. 88, Middle Haixi Road, Jiaonan City, Qingdao, China
Nanjing Vincero International Trading Co.,Ltd
Contact:8618936897229
Address:NO.68, ZhuShan Road, JiangNing WanDa Plaza, Building E Room 1703
Jinan Yijialian Economic and Trade Development Co., Ltd.
Contact:+86 0531-66729596
Address:jinan
Zhejiang Bolaite Technology Co.,Ltd
Contact:+86-0570-6523168 +86-0570-6523150
Address:No.5 YinKeng Road Kaihua Industrial Park(Chengguan Town) Zhejiang Prov.
Lianyungang Ningkang Chemical Co., Ltd
Contact:.+86-518-88588008
Address:http://www.chemnk.com
Doi:10.1039/c1cc14162g
(2011)Doi:10.1007/BF01339608
(1930)Doi:10.1016/j.tet.2016.06.016
(2016)Doi:10.1002/chem.201604415
(2016)Doi:10.1021/ja01479a055
(1961)Doi:10.1134/S1070363210080256
(2010)