FUNGICIDAL AND BACTERICIDAL ACTIVITY OF METAL DIETHYLDITHIOCARBAMATE FUNGICIDES
–
1
(
50, 100 mg mL ) were added. Controls were
maintained with acetone and clotrimazole
(241.48–349.72°C). The results are recorded in
Table 6. It shows good fungicidal and bactericidal ac-
tivity as it inhibits the growth of both fungi and bacte-
ria (Tables 7–9).
–
1
(
100 mg mL ). The treated and the controls were
kept at room temperature for 48 h. Inhibition zones
were measured and diameter was calculated in mil-
limeter. Three to four replicates were maintained
for each treatment.
Lead diethyldithiocarbamate
•
Effect of dithiocarbamates fungicides on the spore
germination: The fungicidal effect of synthesized
metal dithiocarbamates was tested on spore germi-
nation of Bipolaris sorokinina. The test was based
on the slide-germination technique [20] using a
The lead diethyldithiocarbamate under study is off
white powdered substance. It is soluble in 1 M HCl,
1 M HNO
, 1 M H SO and benzene and insoluble in
3 2 4
distilled water, deep well water, 1 M NaOH,
1 M K
2
Cr
2
O
7
(oxidizing agent) and 1 M ascorbic acid,
and 1 M hydroxyl amine (reducing
–
concentration 2000 ppm mL of dithiocarbamates
1
1 M Na
S
O
4
2
2
under study.
agent). The results are recorded in Table 2. It melts at
–
1
2
24°C (Table 3). IR spectrum shows the peak (cm )
at 1483.07s, 1456.04w, 1417.57s, 1376.62w,
353.09w, 1298.86w, 1265.85s, 1204.07w,
137.14m, 1070.81sh, 980.85sh, 905.35w. The X-ray
Bacterial activity test
1
The synthesized metal dithiocarbamates were tested
for bactericidal activity by broth dilution method [21]
using six test organisms: Bacillus subtilis, Staphylo-
coccus aureus, Pseudomonas aeruginosa, E. coli, Ba-
cillus sphaericus, Chromobacterium violaceum. The
broth dilution method is as follows:
1
diffraction data are reported in Table 5. DTA and TG
grams show only one peak at 345°C and only one pla-
teau corresponding to 52.78% conversion
(
241.48–349.72°C). The results are recorded in Ta-
ble 6. It shows good fungicidal and bactericidal activ-
ity as it inhibits the growth of both fungi and bacteria
(Tables 7–9).
Broth dilution method
The test was used to determine the minimal inhibitory
concentration (MIC). Serial dilutions of a stock solu-
tion of compounds were incorporated into 0.5 mL of
nutrient broth in glass tubes to get required concentra-
tion. All the tubes were inoculated with standardized
inoculum of organism. Control was maintained with
acetone in the last tube. The whole setup was incu-
bated at 25–30°C for 8 days. Examine tubes for visi-
ble signs of bacterial growth. The lightest dilution
without growth is the minimal inhibitory concentra-
tion (MIC).
Cadmium diethyldithiocarbamate
The cadmium diethyldithiocarbamate under study is
white powdered substance. It is soluble in 1 M HCl,
1
M HNO
in 1 M NaOH and insoluble in distilled water, deep
well water, 1 M K Cr (oxidizing agent) and 1 M
ascorbic acid, 1 M Na S O and 1 M hydroxyl amine
3 2 4
, 1 M H SO and benzene; slightly soluble
O
2 7
2
2
2
4
(
reducing agent). The results are recorded in Table 2.
It melts at 280°C (Table 3). IR spectrum (Table 4)
–1
shows the peak (cm ) at 1502.64s, 1454.26w,
1
1
9
430.29s, 1377.28w, 1352.37w, 1297.91w, 1271.61s,
206.89s, 1145.24m, 1096.62w, 1071.67m, 991.34s,
10.34m. The X-ray diffraction data are reported in
Results and discussion
Copper diethyldithiocarbamate
Table 5. DTA and TG curves show only one peak at
61°C and only one plateau corresponding to 81.84%
3
Copper diethyldithiocarbamate under study is dark
brown powdered substance. It is soluble in 1 M HCl,
conversion (241.48–349.72°C). The results are re-
corded in Table 6. It shows good fungicidal and bac-
tericidal activity as it inhibits the growth of both fungi
and bacteria (Tables 7–9).
1
M HNO
, 1 M H SO and benzene and insoluble in
3 2 4
distilled water, deep well water, 1 M NaOH,
1
M K
2
Cr
2
O
7
(oxidizing agent) and 1 M ascorbic acid,
and 1 M hydroxyl amine (reducing
1
M Na
2
S
2
O
4
Nickel diethyldithiocarbamate
agent). The results are recorded in Table 2. It melts at
–
1
2
10°C (Table 3). IR spectrum shows the peak (cm )
at 1675.56w, 1506.00s, 1431.71sh, 1376.66sh,
351.59sh, 1299.59w, 1271.72s, 1205.00w,
145.09w, 1098.25w, 1070.99w, 993.10m, 910.07w.
The nickel diethyldithiocarbamate under study is
green powder. It is soluble in 1 M HCl, 1 M HNO
M H SO and benzene and insoluble in distilled wa-
ter, deep well water, 1 M NaOH, 1 M K Cr (oxi-
dizing agent) and 1 M ascorbic acid, 1 M Na S O and
3
,
1
1
2
4
1
O
2 7
2
X-ray diffraction data are reported in Table 5. DTA
and TG curves show two peaks at 247 and 320°C and
only one plateau corresponding to 73.63% conversion
2
2
4
1
M hydroxyl amine(reducing agent). The results are
J. Therm. Anal. Cal., 94, 2008
77