Analytical Chemistry
Page 6 of 8
(13) Takeda, A.; Tamano, H. Front. Aging Neurosci. 2014, 6, 26-
32.
(14) Dou, M.; Gong, A.; Liang, H.; Wang, Q.; Wu, Y.; Ma, A.; Han, L.
Neurosci. Lett. 2018, 683, 196-201.
(15) Frederickson, C. J.; Koh, J. Y.; Bush, A. I. Nat. Rev. Neurosci.
2005, 6, 449-462.
(16) Traynelis, S. F.; Cull-Candy, S. G. J. Physiol. 1991, 433, 727-
763.
(17) Yao, H.; Haddad, G. G. Cell Calcium 2004, 36, 247-255.
(18) Waldmann, R.; Lazdunski, M. Curr. Opin. Neurobiol. 1998, 8,
418-424.
(19) Schumacher, M. A. Pain Pract. 2010, 10, 185-200.
(20) Jalali-Yazdi, F.; Chowdhury, S.; Yoshioka, C.; Gouaux, E. Cell
2018, 175, 1520-1532.
(21) Paoletti, P.; Ascher, P.; Neyton, J. J. Neurosci. 1997, 17, 5711-
5725.
(22) Traynelis, S. F.; Hartley, M.; Heinemann, S. F. Science 1995,
268, 873-876.
(23) Chen, B.; Liu, J.; Yang, T.; Chen, L.; Hou, J.; Feng, C.; Huang, C.
Z. Talanta 2019, 191, 357-363.
(24) Liu, Y.; Fares, M.; Dunham, N. P.; Gao, Z.; Miao, K.; Jiang, X.;
Bollinger, S. S.; Boal, A. K.; Zhang, X. Angew. Chem. Int. Ed. 2017, 56,
8672-8676.
(25) Ning, J.; Wang, W.; Ge, G.; Chu, P.; Long, F.; Yang, Y.; Peng, Y.;
Feng, L.; Ma, X.; James, T. D. Angew. Chem. Int. Ed. 2019, 58, 9959-
9963.
(26) Khan, M.; Goldsmith, C. R.; Huang, Z.; Georgiou, J.; Luyben,
T. T.; Roder, J. C.; Lippard, S. J.; Okamoto, K. Natl. Acad. Sci. U. S. A.
2014, 111, 6786-6791.
(27) Zhu, H.; Fan, J.; Zhang, S.; Cao, J.; Song, K.; Ge, D.; Dong, H.;
Wang, J.; Peng, X. Biomater. Sci. 2014, 2, 89-97.
(28) Tang, B.; Yu, F.; Li, P.; Tong, L.; Duan, X.; Xie, T.; Wang, X. J.
Am. Chem. Soc. 2009, 131, 3016-3023.
(29) Lee, D.; Swamy, K. M. K.; Hong, J.; Lee, S.; Yoon, J. Sens.
Actuators, B 2018, 266, 416-421.
(30) Yamada, T.; Fujiwara, S.; Fujita, K.; Tsuchido, Y.; Hashimoto,
T.; Hayashita, T. Molecules 2018, 23, 635-646.
(31) Chen, T.; Pei, X.; Yue, Y.; Huo, F.; Yin, C. Spectrochim. Acta,
Part A 2019, 209, 223-227.
In summary, we developed a robust fluorescent probe
DNP for simultaneously monitoring Zn2+ and H+ in mouse
brains. Utilizing DNP, we mapped the increased Zn2+ and H+
in PC12 cells under the oxidative stress. Of note, in vivo
imaging for the first time revealed the simultaneous
reduction of Zn2+ and pH in brains of mice with depression-
like behaviors. Further results implied that the NMDA
receptor might be responsible for the coinstantaneous
fluctuation of Zn2+ and H+ during depression. Altogether,
this work provides strong and direct evidence for disclosing
the relationships between Zn2+, H+ and depression,
advancing our understanding of the pathogenesis during
depression.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
ASSOCIATED CONTENT
Supporting Information
The Supporting Information is available free of charge on the
ACS Publications website.
Additional
experimental
data,
including
synthesis,
characterization, photophysical properties, cytotoxicity, and
experimental details.
AUTHOR INFORMATION
Corresponding Author
*E-mail: lip@sdnu.edu.cn
*E-mail: tangb@sdnu.edu.cn
ORCID
Bo Tang: 0000-0002-8712-7025
Author Contributions
‡These authors contributed equally.
ACKNOWLEDGMENT
(32) Wu, L.; Li, X.; Ling, Y.; Huang, C.; Jia, N. ACS Appl. Mater.
Interfaces 2017, 9, 28222−28232.
(33) Han, J.; Burgess, K. Chem. Rev. 2010, 110, 2709–2728.
(34) Lee, L. G.; Berry, G. M.; Chen, C. H. Cytometry 1989, 10, 151-
164.
(35) Xu, Z.; Yoon, J.; Spring, D. R. Chem. Soc. Rev. 2010, 39, 1996-
2006.
(36) Zhang, W.; Wang, X.; Li, P.; Xiao, H.; Zhang, W.; Wang, H.;
This work was supported by the National Natural Science
Foundation of China (21675105, 21535004, 91753111,
21927811), Key Research and Development Program of
Shandong Province (2018YFJH0502), National Major Scientific
and Technological Special Project for “Significant New Drugs
Development” (2017ZX09301030004), and Natural Science
Foundation of Shandong Province of China (ZR2017ZC0225).
Tang, B. Anal. Chem. 2017, 89, 6840-6845.
REFERENCES
(37) Coyle, J. T.; Puttfarcken, P. Science 1993, 262, 689-695.
(38) Murphy, T. H.; Miyamoto, M.; Sastre, A.; Schnaar, R. L.; Coyle,
J. T. Neuron 1989, 2, 1547–1558.
(39) Lee, H. Y.; Weon, J. B.; Ryu, G.; Yang, W. S.; Kim, N. Y.; Kim,
M. K.; Ma, C. J.; BMC Complementary Altern. Med. 2017, 17, 207–
213.
(1) Kessler, R. C.; Berglund, P.; Demler, O.; Jin, R.; Merikangas, K.
R.; Walters, E. E. Arch. Gen. Psychiatry 2005, 62, 768-768.
(2) Nestler, E. J.; Barrot, M.; DiLeone, R. J.; Eisch, A. J.; Gold, S. J.;
Monteggia, L. M. Neuron 2002, 34, 13-25.
(3) Krishnan, V.; Nestler, E. J. Nature 2008, 455, 894-902.
(4) Carroll, R. C.; Zukin, R. S. Trends Neurosci. 2002, 25, 571-577.
(5) Lau, C. G.; Zukin, R. S. Nat. Rev. Neurosci. 2007, 8, 413-426.
(6) McCarthy, D. J.; Alexander, R.; Smith, M. A.; Pathak, S.; Kanes,
S.; Lee, C.-M.; Sanacora, G. Med. Hypotheses 2012, 78, 675-681.
(7) Valentine, G. W.; Sanacora, G. Biochem. Pharmacol. 2009, 78,
431-439.
(8) Papp, M.; Moryl, E. Eur. J. pharmacol. 1994, 263, 1-7.
(9) Skolnick, P.; Layer, R. T.; Popik, P.; Nowak, G.; Paul, I. A.;
Trullas, R. Pharmacopsychiatry 1996, 29, 23-26.
(10) Yang, Y.; Cui, Y.; Sang, K.; Dong, Y.; Ni, Z.; Ma, S.; Hu, H. Nature
2018, 554, 317-322.
(40) Lagadic-Gossmann, D.; Huc, L.; Lecureur, V. Cell Death
Differ. 2004, 11, 953-961.
(41) Mulkey, D. K.; Henderson, R. A.; Ritucci, N. A.; Putnam, R. W.;
Dean, J. B. Am. J. Physiol. Cell Physio. 2004, 286, C940-C951.
(42) Niu, J.; Fan, J.; Wang, X.; Xiao, Y.; Xie, X.; Jiao, X.; Sung, C.;
Tang, B. Anal. Chem. 2017, 89, 7210-7215.
(43) Moda-Sava, R. N.; Murdock, M. H.; Parekh, P. K.; Fetcho, R.
N.; Huang, B. S.; Huynh, T. N.; Witztum, J.; Shaver, D. C.; Rosenthal,
D. L.; Alway, E. J.; Lopez, K.; Meng, Y.; Nellissen, L.; Grosenick, L.;
Milner, T. A.; Deisseroth, K.; Bito, H.; Kasai, H.; Liston, C. Science
2019, 364, 147-158.
(44) Gourley, S. L.; Wu, F. J.; Kiraly, D. D.; Ploski, G. E.; Kedves, A.
T.; Duman, R. S.; Taylor. J. R. Biol. Psychiatry 2008, 63, 353-359.
(45) Blendy, J. A. Biol. Psychiatry 2006, 59, 1144–1150.
(11) Tamano, H.; Takiguchi, M.; Shimaya, R.; Adlard, P. A.; Bush,
A. I.; Takeda, A. Biochem. Biophs. Res. Commun. 2019, 514, 888-892.
(12) Sindreu, C.; Palmiter, R. D.; Storm, D. R. Proc. Natl. Acad. Sci.
U. S. A. 2011, 108, 3366-3370.
ACS Paragon Plus Environment