Tetrahedron Letters p. 7279 - 7282 (1998)
Update date:2022-08-25
Topics:
Marcaurelle, Lisa A.
Bertozzi, Carolyn R.
An amino acid bearing an unprotected ketone group, (2S)-aminolevulinic acid, was incorporated into a synthetic peptide using standard Fmoc-based solid-phase methods. The ketone group remained unharmed during the synthesis and provided a uniquely reactive functional group for covalent modification of the peptide. The ketone and the sulfhydryl group of a cysteine residue elsewhere in the peptide were reacted simultaneously with two different biophysical probes, enabling the site-specific installation of a donor and acceptor pair for FRET in one step without the need for differential side chain protection.
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(2017)Doi:10.1016/S0022-328X(00)91079-9
(1967)Doi:10.1016/S0020-1693(00)83355-1
(1988)Doi:10.1016/S0040-4039(97)00284-0
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