4190
Med Chem Res (2013) 22:4183–4191
Dickschat JS (2010) Quorum sensing and bacterial biofilms. Nat Prod
Rep 27:343–369
ethanol solutions (10–100 %). The samples were dried,
mounted on aluminum stubs with conductive carbon
cement, and then coated with a gold film. Samples were
observed with a Hitachi 14800 SEM at 1.5 kV.
Donlan RM, Costerton JW (2002) Biofilms: survival mechanisms of
clinically relevant microorganisms. Clin. Microbiol Rev 15:167–193
Dwyer DJ, Kohanski MA, Collins JJ (2009) Role of reactive oxygen
species in antibiotic action and resistance. Curr Opin Microbiol
12:482–489
Geske GD, Wezeman RJ, Siegel AP, Blackwell HE (2005) Small
molecule inhibitors of bacterial quorum sensing and biofilm
formation. J Am Chem Soc 127:12762–12763
Quantification of biofilm
P. aeruginosa was grown overnight in LB medium at
37 °C with agitation. After growth, the culture was diluted
with LB medium (OD600 0.02), and 50 ll of the diluted
culture was added to 940 ll of LB medium supplemented
with 6.25–100 lg/ml of MPAT and were incubated stati-
cally for 18 h at 37 °C in 24-well polystyrene plates. After
incubation, planktonic bacteria were discarded, and the
biofilms were washed three times with phosphate buffered
saline buffer. Washed biofilms were fixed with 1 ml of
methanol (99 %). After 15 min, the methanol was dis-
carded, and the plates were dried at room temperature.
Crystal violet (0.1 % in water) was then added to each well
(1 ml/well), and the plates were incubated for 15 min at
room temperature. Crystal violet was then discarded, and
stained biofilms were washed three times with 1 ml of
water. Acetic acid (33 % in water) was added to the stained
biofilms (2 ml) to solubilize the crystal violet, and the
absorbance of the solution was read at 590 nm with a
Shimadzu spectrophotometer (Japan).
Givskov M, De NysR, Manefield M, Gram L, Maximilien R, Eberl L,
Molin S, Steinberg PD, Kjelleberg S (1996) Eukaryotic inter-
ference with homoserine lactone-mediated prokaryotic signaling.
J Bacteriol 178:6618–6622
Gonzalez JE, Keshavan ND (2006) Messing with bacterial quorum
sensing. Microbiol Mol Biol Rev 70:859–875
Gram L, de Nys R, Maximilien R, Givskov M, Steinberg P,
Kjelleberg S (1996) Inhibitory effects of secondary metabolites
from the red alga Deliseapulchraon swarming motility of
Proteus mirabilis. Appl Environ Microbiol 62:4284–4287
Hammer BK, Bassler BL (2003) Quorum sensing controls biofilm
formation in Vibrio cholerae. Mol Microbiol 50:101–104
Hentzer M, Wu H, Andersen JB, Rasmussen RK, TB BN, Kumar N,
Schembri MA, Song Z, Kristoffersen P, Manefield M, Costerton
JW, Molin S, Eberl L, Steinberg P, Kjelleberg S, Høiby N,
Givskov M (2005) Attenuation of Pseudomonas aeruginosa
virulence by quorum sensing inhibitors. EMBO J 22:3803–3815
Horowitz HH, Metzger G (1963) A new analysis of thermogravimet-
ric traces. Anal Chem 35:1464–1468
Imlay JA (2003) Pathways of oxidative damage. Annu Rev Microbiol
57:395–418
Ines Albesa M, Becerra C, Battan PC, Paez PL (2004) Oxidative
stress involved in the antibacterial action of different antibiotics.
Biochem Biophys Res Commun 317:605–609
Ishida T, Ikeda T, Takiguchi NA, Kuroda AH, Ohtake H, J Kato
(2007) Inhibition of quorum sensing in Pseudomonas aeruginosa
by N-acyl cyclopentylamides. Appl Environ Microbiol 73:
3183–3188
Acknowledgments Authors are thankful to the authorities of
Solapur University, University of Pune and Vidya Pratishthan’s,
A.S.C.College Baramati, for providing research facilities.
Kaplancikli ZA, Zitouni GT, Revial G, Guven K (2004) Synthesis
and study of antibacterial and antifungal activities of novel
2-[[(benzoxazole/benzimidazole-2-yl)sulfanyl] acetylamino] thi-
azoles. Arch Pharm Res 27:1081–1085
Karade HN, Acharya BN, Manisha S, Kaushik MP (2008) Design,
synthesis, and antimalarial evaluation of thiazole-derived amino
acids. Med Chem Res 17:19–29
References
Al-Saddi MS, Faidallah HM, Rostom SAF (2008) Synthesis and
biological evaluation of some 2,4,5-trisubstituted thiazole deriv-
atives as potential antimicrobial and anticancer agents. Archived
der pharmazie 341:424–434
Karpov KA, Nazarenko AV, Pekarevskii BV, Potekhin VM (2001)
Biocidal and anticorrosive effect of aminothiazole derivatives
used as additives to jet fuels. Russ J Appl Chem 74:998–1001
Kovacic JE (1967) The CN stretching frequency in the infrared
spectra of Schiff’s base complexes-I. Copper complexes of
salicylidene anilines. Spectochim Acta 23A:183–187
Leone S, CesareMutti AK, Mattia Sturlese SM, Cattaneo E,
DoroteaRigamontib AC (2008) SAR and QSAR study on
2-aminothiazole derivatives, modulators of transcriptional
repression in Huntington’s disease. Bioorg Med Chem 16:5695–5703
MacCallum JR, Tanner J (1970) A comparative study of some
methods of assessing kinetic parameters from thermogravimetric
analysis. Euro Poly J 6:907–917
Manefield M, de Nys R, Kumar N, Read R, Givskov M, Steinberg P,
Kjelleberg S (1999) Evidence that halogenated furanones from
Deliseapulchra inhibit acylatedhomoserine lactone (AHL)-med-
iated gene expression by displacing the AHL signal from its
receptor protein. Microbiology 145:283–291
Mathews RA, Gregory JT (1952) Reactions of 3-thiocyano-
2-butanone. II.1 The preparation of 2-arylamino- and 2-alkyl-
amino-4,5-dimethylthiazoles. J Am Chem Soc 74:3867–3868
Baselt T, Rehse K (2008) New 2-amino-thiazole-4-acetamides with
antiplatelet activity. Archived der pharmazie 341:645–654
Bjarnsholt T, Givskov M (2008) Quorum sensing inhibitory drugs as
next generation antimicrobials: worth the effort? Curr Infect Dis
Rep 10:22–28
Coats AW, Reddfern JP (1964) Kinetic parameters from thermo-
gravimetric data. Nature 201:68–69
Convover LH, Tarbell DS (1950) Thiazole analogs of pyridoxine.
J Am Chem Soc 72:5221–5225
Costerton JW, Stewart PS, Greenberg EP (1999) Bacterial biofilms: a
common cause of persistent infections. Science 284:1318–1322
Cvitkovitch DG, Li YH, Ellen RP (2003) Quorum sensing and biofilm
formation in Streptococcal infections. J Clin Invest 112:1626–1632
Dash B, Raut MK (1955) Sulphonamide derivatives of thiazoles
Indian. J Chem Soc 32:663
Davies J (1994) Inactivation of antibiotics and the dissemination of
resistance genes. Science 264:375–382
Davies D (2003) Understanding biofilm resistance to antibacterial
agents. Nat Rev Drug Discov 2:114–122
Davies J (2006) Where have all the antibiotics gone? Can J Infect Dis
Med Microbiol 17:287–290
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