Fluorescamine

Base Information

• Chemical Name: Fluorescamine
• CAS No.: 38183-12-9
• Molecular Formula: C17H10O4
• Molecular Weight: 278.264
• Hs Code.: 29322090
• European Community (EC) Number: 253-814-5
• UNII: Y6859V58YW
• DSSTox Substance ID: DTXSID50872017
• Nikkaji Number: J22.287I
• Wikipedia: Fluorescamine
• Wikidata: Q238913
• Mol file: 38183-12-9.mol

Synonyms:Fluorescamine;Fluram

Chemical Property of Fluorescamine

Chemical Property:

• Appearance/Colour: white powder
• Vapor Pressure: 3.71E-12mmHg at 25°C
• Melting Point: 155-157 °C
• Refractive Index: 1.686
• Boiling Point: 550.3 °C at 760 mmHg
• Flash Point: 248.5 °C
• PSA: 52.60000
• Density: 1.43 g/cm³
• LogP: 2.65020
• Storage Temp.: 2-8°C
• Solubility.: acetonitrile: soluble
• Water Solubility.: Soluble in acetone, ethanol, chloroform, dimethylsulfoxide and acetonitrile. Slightly soluble in water.
• XLogP3: 2.9
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 1
• Exact Mass: 278.05790880
• Heavy Atom Count: 21
• Complexity: 503

Purity/Quality:

98%,99%, ^*data from raw suppliers

Fluorescamine ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xi, F
• Hazard Codes: F,Xi
• Statements: 11-36/38-66-67-36-36/37/38
• Safety Statements: 22-24/25-36/37-33-29-26-16-9-37/39

MSDS Files:

SDS file from LookChem

Useful:

• Chemical Classes: Dyes -> Indicator Dyes
• Canonical SMILES: C1=CC=C(C=C1)C2=COC3(C2=O)C4=CC=CC=C4C(=O)O3
• Uses: Fluorescamine is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Reaction takes place under mild conditions. Low background due to hydrolysis. For fluorescent detection of amino acids, peptides and proteins.Fluorescamine plays an important role as a reagent for the detection of amines, peptides and proteins. It is also used as a substrate for measuring protease activity. It is utilized in the detection and quantitation of residual aminopenicillins and sulfonamides in honey by HPLC. It finds application in the determination of lisinopril in human plasma and urine.

Technology Process of Fluorescamine

There total 2 articles about Fluorescamine which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 96.0%

C17H12O4 → fluorescamine (38183-12-9)

Guidance literature:

With sodium periodate; In water; at 20 ℃; for 8h; Reagent/catalyst; Large scale;

Reference yield:

→ fluorescamine (38183-12-9)

Guidance literature:

DOI:10.1021/ja00771a084

Reference yield: 77.0%

7α-(4-Aminobutyl)-3,17β-estradiol (102035-85-8) + fluorescamine (38183-12-9) → 1'-(Estra-1,3,5(10)-trien-3,17β-diol-7α-yl)-butyl-3'-phenyl-5'-(2'-carboxyphenyl)-5'-hydroxy-2'-pyrrolin-4'-on

Guidance literature:

In tetrahydrofuran; for 1h;

DOI:10.1002/ardp.19863190303

Downstream raw materials:

6β-{(R)-2-[(Ξ)-2-(2-carboxy-phenyl)-2-hydroxy-3-oxo-4-phenyl-2,3-dihydro-pyrrol-1-yl]-2-phenyl-acetylamino}-penicillanic acid

5-(2-carboxyphenyl)-5-hydroxy-1-(4-methylphenyl)-3-phenyl-2-pyrrolin-4-one

5-(2-carboxyphenyl)-1-(4-chlorophenyl)-5-hydroxy-3-phenyl-2-pyrrolin-4-one

5-(2-carboxyphenyl)-5-hydroxy-1-(4-methoxyphenyl)-3-phenyl-2-pyrrolin-4-one

Refernces

• 1、 -. "Enkephalin analogues". . . Retrieved 2008/06/13.
• 2、 -. "Spirolactone production". . . Retrieved 2008/06/13.