5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Base Information

• Chemical Name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
• CAS No.: 6578-06-9
• Molecular Formula: C8H6BrClNO4P^.C7H9N
• Molecular Weight: 433.626
• Hs Code.: 29339190
• European Community (EC) Number: 229-506-1
• DSSTox Substance ID: DTXSID0064412
• Mol file: 6578-06-9.mol

Synonyms:6578-06-9;BCIP;5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt;BCIP p-toluidine salt;X-phosphate p-toluidine salt;p-toluidine 5-bromo-4-chloro-1H-indol-3-yl phosphate;5-Bromo-4-chloro-1H-indol-3-yl phosphate p-toluidine;5-Bromo-4-chloro-3-indolyl phosphate, p-toluidine salt;MFCD00040636;SCHEMBL1474652;DTXSID0064412;EINECS 229-506-1;5-Bromo-4-chloro-3-indolyl hydrogen phosphate - p-toluidine (1:1);AKOS015902727;FD10002;AC-37057;HY-15909;B1239;FT-0620130;FT-0620131;5-Bromo-4-chloro-3-indolyl phosphate, p-toluidinesalt;5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, tablet;BCIP, 5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt;5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, >=95.0% (HPLC);5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, >=99% (HPLC);5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, BioReagent, for molecular biology, powder, >=99%;BCIP p-toluidine;X-Phosphate p-toluidine;Phosphoric acid-mono-(5-bromo-4-chloro-1H-indol-3-yl) ester p-toluidine

Chemical Property of 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

Chemical Property:

• Appearance/Colour: crystals
• Vapor Pressure: 2.65E-14mmHg at 25°C
• Melting Point: 194-195 °C
• Boiling Point: 676.2 °C at 760 mmHg
• Flash Point: 362.7 °C
• PSA: 118.38000
• LogP: 5.21370
• Storage Temp.: 2-8°C
• Solubility.: DMF: 20 mg/mL
• Water Solubility.: DMF: 20 mg/mL
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 2
• Exact Mass: 431.96413
• Heavy Atom Count: 24
• Complexity: 377

Purity/Quality:

99% ^*data from raw suppliers

5-Bromo-4-chloro-3-indolyl-phosphate4-ToluidineSalt ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xi
• Hazard Codes: Xi
• Statements: 36/37/38-40
• Safety Statements: 26

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Chemical Classes: Nitrogen Compounds -> Indoles
• Canonical SMILES: CC1=CC=C(C=C1)N.C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br
• Uses: For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Technology Process of 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

There total 1 articles about 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

→ 5-bromo-4-chloro-3-indolyl phospate p-toluidine salt (6578-06-9)

Guidance literature:

Reference yield: 99.0%

morpholine (110-91-8,99108-56-2) + 5-bromo-4-chloro-3-indolyl phospate p-toluidine salt (6578-06-9) + dicyclohexyl-carbodiimide (538-75-0) → 4-morpholinyl-N,N'-dicyclohexylcarboxamidinium 5-bromo-4-chloro-3-indolyl phosphomorpholidate

Guidance literature:

In 2-methyl-propan-1-ol; water; for 15h; Heating;

DOI:10.1016/j.bmcl.2005.12.016

Refernces