Deoxyribonuclease

Base Information

• Chemical Name: Deoxyribonuclease
• CAS No.: 9003-98-9
• Molecular Formula: NULL
• Molecular Weight: 0
• Hs Code.: 35079090
• Mol file: 9003-98-9.mol

Synonyms:AlkalineDNase;Alkaline deoxyribonuclease;DNA depolymerase;DNA endonuclease;DNAnuclease;DNAase;DNase;DNase I;DNase Y;DNase g;Deoxyribonuclease;Deoxyribonuclease (pancreatic);Deoxyribonuclease A;Deoxyribonucleic phosphatase;Desoxyribonuclease;Dornase;Dornava;Dornavac;E.C. 3.1.21.1;E.C. 3.1.4.5;Endodeoxyribonuclease I;Endonuclease DNAS1L3;Endonuclease S;Escherichia coliEndonuclease I;NUC18 nuclease;Nuclease, Escherichia coli endo-, I;Pancreaticdeoxyribonuclease;Pancreatic dornase;

Chemical Property of Deoxyribonuclease

Chemical Property:

• Appearance/Colour: white solution (clear, colorless)
• Vapor Pressure: 0.004Pa at 25℃
• PSA: 0.00000
• Density: 100 g/mL at 20 °C
• LogP: 0.00000
• Storage Temp.: −20°C
• Solubility.: Soluble in 0.15 M sodium chloride (5.0mg/L) clear.
• Water Solubility.: 125g/L at 25℃

Purity/Quality:

99% ^*data from raw suppliers

DNASE1 ^*data from reagent suppliers

Safty Information:

• Safety Statements: 23-24/25-22

MSDS Files:

SDS file from LookChem

Useful:

• Uses: Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples. Amplification grade DNase I has been used for the digestion of DNA during isolation and purification of RNA. The purified RNA can be used for the synthesis of cDNA using RNA reverse transcriptase.
• Clinical Use: DNAse is a human endonuclease, normally present insaliva, urine, pancreatic secretions, and blood. The enzymecatalyzes the hydrolysis of extracellular DNA into oligonucleotides.Aerosolized recombinant human deoxyribonucleaseI (rhDNAse), dornase alfa, Pulmozyme, has beenformulated into an inhalation agent for the treatment of pulmonarydisease in patients with CF.Among the clinical manifestations of CF are obstructionof the airways by viscous, dehydrated mucus. Pulmonaryfunction is diminished, and microbes can become entrappedin the viscid matrix. A cycle of pulmonary obstruction andinfection leads to progressive lung destruction and eventualdeath before the age of 30 for most CF patients. The immunesystem responds by sending in neutrophils, and theseaccumulate and eventually degenerate, releasing largeamounts of DNA. The high levels of extracellular DNA releasedand the mucous glycoproteins are responsible for thedegenerating lung function. The DNA-rich secretions alsobind to aminoglycoside antibiotics typically used to treat theinfections. In vitro studies showed that the viscosity of thesecretions could be reduced by application of DNAse I.Pulmozyme is approved for use in the treatment of CFpatients, in conjunction with standard therapies, to reducethe frequency of respiratory infections requiring parenteralantibiotics and to improve pulmonary function. The dose isdelivered at a level of 2.5 mg daily with a nebulizer.Pulmozyme is not a replacement for antibiotics, bronchodilators,and daily physical therapy.