Calcein AM

Base Information

• Chemical Name: Calcein AM
• CAS No.: 148504-34-1
• Molecular Formula: C46H46 N2 O23
• Molecular Weight: 762.717
• NSC Number: 689290
• DSSTox Substance ID: DTXSID101043565
• Nikkaji Number: J674.668C
• Wikidata: Q27068143
• Metabolomics Workbench ID: 68485
• ChEMBL ID: CHEMBL1977168
• Mol file: 148504-34-1.mol

Synonyms:4',5'-bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester;calcein AM;calcein-acetoxymethyl ester;calcein-AM

Chemical Property of Calcein AM

Chemical Property:

• Vapor Pressure: 0mmHg at 25°C
• Melting Point: >250℃
• Refractive Index: n20/D 1.479
• Boiling Point: 982.7°Cat760mmHg
• PKA: 2.66±0.50(Predicted)
• Flash Point: 548.1°C
• PSA: 305.01000
• Density: 1.49g/cm³
• LogP: 1.96440
• Storage Temp.: −20°C
• Solubility.: DMSO: soluble10mg/mL, clear, colorless to slightly yellow
• XLogP3: 2.9
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 25
• Rotatable Bond Count: 32
• Exact Mass: 994.24913572
• Heavy Atom Count: 71
• Complexity: 1810

Purity/Quality:

99% ^*data from raw suppliers

Calcein AM ^*data from reagent suppliers

Safty Information:

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36

MSDS Files:

Total 1 MSDS from other Authors

Useful:

• Canonical SMILES: CC(=O)OCOC(=O)CN(CC1=CC2=C(C=C1OC(=O)C)OC3=C(C24C5=CC=CC=C5C(=O)O4)C=C(C(=C3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
• Uses: Calcein-AM appears to best satisfy the criteria for assaying cell adhesion and to have the least effect on cell viability and cell functions. Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule