PNGASE F

Base Information

• Chemical Name: PNGASE F
• CAS No.: 83534-39-8
• Molecular Weight: 0
• Mol file: 83534-39-8.mol

Synonyms:Amidase,glycopeptide 4-aspartylglycosylamine; Amidase, peptidoglycanaspartylglucosamine; Aspartylglucosamine amidase; E.C. 3.2.2.18; E.C. 3.5.1.52;Glycoamidase; Glycoamidase F; Glycopeptidase F; Glycosidase, glycopeptide N-;N-Glycanase; N-Glycosidase F; PGNase F; PNGase F; Peptide N-glycanase; PeptideN-glycosidase; Peptide N-glycosidase F; Peptide N4-(N-acetyl-b-glucosaminyl)asparagine amidase;Peptide-N4-(N-acetyl-b-D-glucosaminyl)asparagine amidase; Peptide-N4-(N-acetyl-b-glucosaminyl)asparagine amidase;Peptide-N4-(N-acetylglucosaminyl)asparagine amidase; Peptide-N4-(acetyl-b-glucosaminyl)asparagine amidase

Chemical Property of PNGASE F

Chemical Property:

• PSA: 0.00000
• LogP: 0.00000
• Storage Temp.: 2-8°C

Purity/Quality:

98%,99%, ^*data from raw suppliers

PNGase F ^*data from reagent suppliers

Safty Information:

• Hazard Codes: Xi,N,Xn
• Statements: 37/38-41-50/53-43-20/21/22
• Safety Statements: 22-24/25-36/37/39-26-61

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Uses: Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan. PNGase F from Elizabethkingia meningoseptica has been used:for de-N-glycosylation of Zika20virus E proteinto evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cellsto verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA) Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.