5,10-methylenetetrahydrofolate

Base Information

• Chemical Name: 5,10-methylenetetrahydrofolate
• CAS No.: 3432-99-3
• Molecular Formula: C₂₀H₂₃N₇O₆
• Molecular Weight: 457.446
• Mol file: 3432-99-3.mol

Synonyms:Glutamicacid, N-[p-(3-amino-5,6,6a,7-tetrahydro-1-hydroxyimidazo[1,5-f]pteridin-8(9H)-yl)benzoyl]-,L- (8CI); Imidazo[1,5-f]pteridine, L-glutamic acid deriv.;(+)-5,10-Methylene-5,6,7,8-tetrahydrofolic acid;5,10-Methylene-(6RS)-tetrahydrofolic acid;5,10-Methylene-5,6,7,8-tetrahydrofolic acid; 5,10-Methylenetetrafolate;5,10-Methylenetetrahydrofolic acid; CoFactor; Folic acid,tetrahydro-N5,N10-methylene-; Folitixorin;N5,N10-Methylene-5,6,7,8-tetrahydrofolic acid; N5,N10-Methylenetetrahydrofolicacid; N5,N10-Methylenetetrahydropteroylglutamic acid

Chemical Property of 5,10-methylenetetrahydrofolate

Chemical Property:

• PKA: 3.47±0.10(Predicted)
• PSA: 194.97000
• Density: 1.75g/cm³
• LogP: 0.47940
• Storage Temp.: Hygroscopic, -86°C Freezer, Under inert atmosphere
• Solubility.: DMSO (Slightly), Methanol (Slightly, Sonicated)

Purity/Quality:

99%, ^*data from raw suppliers

Folitixorin(~80%)(MixtureofDiastereomers) ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Uses: 5,10-methylenetetrahydrofolate is a stable folate lyophilized compound suitable for use in the treatment of cancer and other therapies.

Technology Process of 5,10-methylenetetrahydrofolate

There total 2 articles about 5,10-methylenetetrahydrofolate which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

tetrahydrofolic acid (135-16-0) + formaldehyd (50-00-0,30525-89-4,61233-19-0) → N5,N10-methylene-5,6,7,8-tetrahydrofolate (3432-99-3,7599-43-1,31690-11-6,31690-12-7,64395-42-2)

Guidance literature:

DOI:10.1021/ja01503a043

Reference yield:

tetrahydrofolic acid (135-16-0) + formaldehyd (50-00-0,30525-89-4,61233-19-0) + water (7732-18-5) → N5,N10-methylene-5,6,7,8-tetrahydrofolate (3432-99-3,7599-43-1,31690-11-6,31690-12-7,64395-42-2)

Guidance literature:

Reference yield:

2'-deoxyuridine-5'-monophosphate (964-26-1) + N5,N10-methylene-5,6,7,8-tetrahydrofolate (3432-99-3,7599-43-1,31690-11-6,31690-12-7,64395-42-2) → thymidine 5'-phosphate (365-07-1,15108-71-1,60363-32-8,96744-88-6,132696-16-3,143838-77-1) + 5-(hydroxymethyl)-2′-deoxyuridine-5′-monophosphate (5238-86-8)

Guidance literature:

With formaldehyd; D-gulose; reduced flavin-dependent thymidylate synthase; glucose oxidase; aq. buffer; Enzymatic reaction;

DOI:10.1021/ja2120822

upstream raw materials:

tetrahydrofolic acid

formaldehyd

water

Downstream raw materials:

thymidine 5'-phosphate

5-(hydroxymethyl)-2′-deoxyuridine-5′-monophosphate