Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,2',4',5',7'-tetrabromo-3',6'-dihydroxy-, disodium salt

Base Information

• Chemical Name: Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,2',4',5',7'-tetrabromo-3',6'-dihydroxy-, disodium salt
• CAS No.: 17372-87-1
• Molecular Formula: C20H6Br4Na2O5
• Molecular Weight: 645.881
• Hs Code.: PRICE
• Mol file: 17372-87-1.mol

Synonyms:Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,2',4',5',7'-tetrabromo-3',6'-dihydroxy-, disodium salt

Chemical Property of Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,2',4',5',7'-tetrabromo-3',6'-dihydroxy-, disodium salt

Chemical Property:

• Appearance/Colour: brownish-red powder
• Vapor Pressure: 0Pa at 25℃
• Melting Point: >300 °C
• Boiling Point: 682.3oC at 760mmHg
• PKA: 2.9, 4.5(at 25℃)
• Flash Point: 11°C
• PSA: 81.65000
• Density: 1.02 g/mL at 20 °C
• LogP: 7.59220
• Storage Temp.: Store at RT.
• Solubility.: H2O: 0.1 g/mL, dark red
• Water Solubility.: freely soluble
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 0
• Exact Mass: 670.69619
• Heavy Atom Count: 30
• Complexity: 645

Purity/Quality:

99.9% ^*data from raw suppliers

May-Grunwald Stain ^*data from reagent suppliers

Safty Information:

• Pictogram(s): F, T, Xn, Xi
• Hazard Codes: F,T,Xn,Xi
• Statements: 11-23/24/25-39/23/24/25-52/53-41-22-36-10
• Safety Statements: 7-16-36/37-45-24/25-61-39-26

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C(=C4OC5=C(C(=C(C=C35)Br)O)Br)Br)O)Br.[Na]
• Uses: Eosin is most often used as a counterstain to haematoxylin in H&E (haematoxylin and eosin) staining. H&E staining is one of the most commonly used techniques in histology. Anti Septic Eosin Yellowish noted to reduce the affinity of Mg2+-ATPase for ATP and increase the enzyme affinity for Mg2+ at concentrations of 10-50 ?M. At higher concentrations near 100 ?M the affinity for Mg2+-ATPase for the ion-activator has been shown to be reduced. This compound has also been reported to be a photosensitizer that works as a molecular photoelectrode via catalyzation of the visible-light-driven electron-transfer reaction. Eosin Y is also used to stain structures such as muscle, cytoplasm, and collagen. This agent also enhances fluorescent quenching abilities by forming a complex with polyvinulpyrrolidone.

Technology Process of Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,2',4',5',7'-tetrabromo-3',6'-dihydroxy-, disodium salt

There total 8 articles about Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,2',4',5',7'-tetrabromo-3',6'-dihydroxy-, disodium salt which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 1.0%

(C6H4CH2NHCH2CH2NHCH2CH2NHCH2)2Cu2(C13H2Br4O3C6H4COO)(2+) + glycine (56-40-6,18875-39-3,25718-94-9) → (C6H4CH2NHCH2CH2NHCH2CH2NHCH2)2Cu2(H2NCH2COOH)(4+) + Eosin Y (548-26-5,602-47-1,17372-87-1)

Guidance literature:

titrn. at pH7; almost no restoration of fluorescence was observed upon the addn. of amino acid; fluorescence spectroscopy data;

DOI:10.1021/ja027110l

Reference yield:

L-alanin (56-41-7,25191-17-7,18875-37-1) + (C6H4CH2NHCH2CH2NHCH2CH2NHCH2)2Cu2(C13H2Br4O3C6H4COO)(2+) → (C6H4CH2NHCH2CH2NHCH2CH2NHCH2)2Cu2(CH3CH(NH2)COOH)(4+) + Eosin Y (548-26-5,602-47-1,17372-87-1)

Guidance literature:

titrn. at pH7; no restoration of fluorescence was observed upon the addn. of amino acid; fluorescence spectroscopy data;

DOI:10.1021/ja027110l

Reference yield:

(C6H4CH2NHCH2CH2NHCH2CH2NHCH2)2Cu2(C13H2Br4O3C6H4COO)(2+) + L-phenylalanine (63-91-2,25191-15-5,658-69-5,67675-33-6) → (C6H4CH2NHCH2CH2NHCH2CH2NHCH2)2Cu2(C6H5CH2CH(NH2)COOH)(4+) + Eosin Y (548-26-5,602-47-1,17372-87-1)

Guidance literature:

titrn. at pH7; no restoration of fluorescence was observed upon the addn. of amino acid; fluorescence spectroscopy data;

DOI:10.1021/ja027110l

upstream raw materials:

C₂₄H₂₂N₄⁽²⁺⁾*C₂₀H₆Br₄O₅^(2-)

fluorescein free acid

glycine

L-alanin

Downstream raw materials:

Eosin ethyl ester

Eosin ethyl ester monosodium salt

Refernces

• 1、 Hortala, Marta Ansa,Fabbrizzi, Luigi,Marcotte, Nathalie,Stomeo, Floriana,Taglietti, Angelo. "Designing the selectivity of the fluorescent detection of amino acids: A chemosensing ensemble for histidine". . p. 20 - 21. Retrieved 2007/10/03.
• 2、 Vasudevan, D.,Basha, C. A.. "A Kinetic Study on the Electrochemical Bromination of Fluorescein to Eosin in a Batch Reactor". . p. 165 - 166. Retrieved 2007/10/03.