4',6-Diamidino-2-phenylindole dihydrochloride

Base Information

• Chemical Name: 4',6-Diamidino-2-phenylindole dihydrochloride
• CAS No.: 28718-90-3
• Molecular Formula: C16H17Cl2N5
• Molecular Weight: 350.25
• Hs Code.: 29339900
• Mol file: 28718-90-3.mol

Synonyms:6-Amidino-2-(4-amidinophenyl)indole dihydrochloride;1H-Indole-6-carboximidamide,2-[4-(aminoiminomethyl)phenyl]-, dihydrochloride (9CI);Indole-6-carboxamidine,2-(p-amidinophenyl)-, dihydrochloride (8CI);

Chemical Property of 4',6-Diamidino-2-phenylindole dihydrochloride

Chemical Property:

• Appearance/Colour: Yellow solid
• Melting Point: >300°C (dec.)
• Boiling Point: 545.4 °C at 760 mmHg
• Flash Point: 283.6 °C
• PSA: 115.53000
• Density: 1.41 g/cm³
• LogP: 5.60710
• Storage Temp.: −20°C
• Solubility.: H2O: 10 mg/mL heat or sonication may be required. Solutions

Purity/Quality:

98%,99%, ^*data from raw suppliers

4′,6-Diamidino-2-phenylindole dihydrochloride ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xi, Xn
• Hazard Codes: Xi,Xn
• Statements: 36/37/38-20/21/22
• Safety Statements: 26-36-24/25-22-37/39

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Description: DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.
• Uses: 4',6-Diamidino-2-phenylindole dihydrochloride is used as a cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine.It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. DAPI is used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex and as a cell permeable fluorescent minor groove-binding probe for DNA. For staining DNA in agarose gels Suitable forDNA staining in agarose gelsanalysis of changes in DNA during apoptosisdetection of mycoplasmaphotofootprinting of DNAimmunofluorescent staining of cellsDAPI has been used:-in rapid monitoring of microbial contamination in chromosomal banding techniquein detection of apoptotic cellsin fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNAto stain mature pollen grains(0.5 mg/ml)

Technology Process of 4',6-Diamidino-2-phenylindole dihydrochloride

There total 9 articles about 4',6-Diamidino-2-phenylindole dihydrochloride which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

C30H25N3O2*2ClH → 4′,6-diamidine-2′-phenylindole dihydrochloride (28718-90-3)

Guidance literature:

With ammonium chloride; In ethanol; at 60 ℃; for 6h;

Reference yield:

C32H29N3O2*2ClH → 4′,6-diamidine-2′-phenylindole dihydrochloride (28718-90-3)

Guidance literature:

With ammonium chloride; In ethanol; at 60 ℃; for 6h;

Reference yield:

C20H21N3O2*2ClH → 4′,6-diamidine-2′-phenylindole dihydrochloride (28718-90-3)

Guidance literature:

With ammonium chloride; In ethanol; at 60 ℃; for 6h;

upstream raw materials:

6-iodo-1 H-indole

6-fluoro-1H-indole

6-bromo-1H-indole

1H-Indole-6-carbonitrile

Downstream raw materials:

4,5-dimethoxy-2-nitrobenzyl [(4-{6-[({[(4,5-dimethoxy-2-nitrobenzyl)oxy]carbonyl}amino)(imino)methyl]-1H-indol-2-yl}phenyl)(imino)methyl]carbamate

Refernces