5(6)-Carboxyfluorescein

Base Information

• Chemical Name: 5(6)-Carboxyfluorescein
• CAS No.: 72088-94-9
• Molecular Formula: C21H12 O7
• Molecular Weight: 376.32
• Hs Code.: 29322090
• Mol file: 72088-94-9.mol

Synonyms:5(6)-Carboxyfluorescein;C 194; Carboxyfluorescein; Fluoresceincarboxylic acid

Chemical Property of 5(6)-Carboxyfluorescein

Chemical Property:

• Appearance/Colour: Off-white solid.
• Melting Point: 275 ºC
• Refractive Index: 1.6380 (estimate)
• Boiling Point: 464.9°C (rough estimate)
• PSA: 226.58000
• Density: 1.3418 (rough estimate)
• LogP: 6.72800
• Storage Temp.: Store at 2-8
• Solubility.: DMSO (Sparingly), Methanol (Slightly, Heated)
• Water Solubility.: slightly soluble

Purity/Quality:

99.9% ^*data from raw suppliers

5(6)-Carboxyfluorescein ^*data from reagent suppliers

Safty Information:

• Hazard Codes: Xi,Xn
• Statements: 36-36/37/38-20/21/22
• Safety Statements: 37/39-26-36-22

MSDS Files:

SDS file from LookChem

Useful:

• Description: 5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.
• Uses: A popular probe of determination of intracellular pH. 5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent