AC-IETD-AFC

Base Information

• Chemical Name: AC-IETD-AFC
• CAS No.: 211990-57-7
• Molecular Formula: C31H38 F3 N5 O12
• Molecular Weight: 729.664
• Mol file: 211990-57-7.mol

Synonyms:CASPASE 8 SUBSTRATE 1F, FLUOROGENIC;CASPASE-8 FLUOROGENIC SUBSTRATE;GRANZYME B SUBSTRATE I, COLORIMETRIC;AC-IETD-AFC;AC-ILE-GLU-THR-ASP-7-AMINO-4-TRIFLUORO-METHYLCOUMARIN;AC-ILE-GLU-THR-ASP-AFC;N-ACETYL-ILE-GLU-THR-ASP-(7-AMINO-4-TRIFLUOROMETHYLCOUMARIN);N-ACETYL-ILE-GLU-THR-ASP-7-AMINO-4-TRIFLUOROMETHYLCOUMARIN AFC

Chemical Property of AC-IETD-AFC

Chemical Property:

• Boiling Point: 1113.8±65.0 °C(Predicted)
• PKA: 4.09±0.10(Predicted)
• PSA: 270.54000
• Density: 1.437±0.06 g/cm3(Predicted)
• LogP: 2.11240
• Storage Temp.: -20°C
• Solubility.: Soluble in DMSO

Purity/Quality:

98%Min ^*data from raw suppliers

Ac-IETD-AFC ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Uses: N-Acetyl-Ile-Glu-Thr-Asp-7-amino-4-(trifluoromethyl)coumarin is a fluorogenic substrate for caspases -6, -8, and -10. The IETD sequence is based on the procaspase-3 cleavage site. Residues 172-175 of procaspase-3 are cleaved during apoptosis to produce the p12 subunit and a p20 peptide that is further cleaved to form the p17 subunit of mature caspase 3. Ac-IETD-AFC is a fluorogenic substrate for caspase-8/caspase-3 processing enzyme and granzyme B. It is also a substrate for caspase-10. The IETD sequence is based on caspase-3 proenzyme cleavage site.

Technology Process of AC-IETD-AFC

There total 1 articles about AC-IETD-AFC which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

C43H62F3N5O12 → C31H38F3N5O12 (211990-57-7)

Guidance literature:

With water; trifluoroacetic acid; at 20 ℃; for 1h;

DOI:10.1002/cmdc.200900356

Reference yield:

C31H38F3N5O12 (211990-57-7) → Coumarin 151 (53518-15-3)

Guidance literature:

With human recombinant caspase 8; In dimethyl sulfoxide; Kinetics; aq. buffer; Enzymatic reaction;

DOI:10.1002/cmdc.200900356

Downstream raw materials:

Coumarin 151

Refernces