NHS-FLUORESCEIN

Base Information

• Chemical Name: NHS-FLUORESCEIN
• CAS No.: 135795-62-9
• Molecular Formula: C24H15NO7
• Molecular Weight: 429.386
• Mol file: 135795-62-9.mol

Synonyms:NHS-FLUORESCEIN

Chemical Property of NHS-FLUORESCEIN

Chemical Property:

• Boiling Point: 720.6±70.0 °C(Predicted)
• PKA: 9.05±0.20(Predicted)
• PSA: 114.12000
• Density: 1.58±0.1 g/cm3(Predicted)
• LogP: 3.42910

Purity/Quality:

99% ^*data from raw suppliers

NHS-FLUORESCEIN 95.00% ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

Technology Process of NHS-FLUORESCEIN

There total 2 articles about NHS-FLUORESCEIN which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 31.0%

1-hydroxy-pyrrolidine-2,5-dione (6066-82-6) + fluorescein free acid (518-45-6) → 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid 2,5-dioxopyrrolidin-1-yl ester (135795-62-9)

Guidance literature:

With dicyclohexyl-carbodiimide; In N,N-dimethyl-formamide; at 70 - 80 ℃; for 1h;

DOI:10.1021/ac020368+

Reference yield:

1-hydroxy-pyrrolidine-2,5-dione (6066-82-6) + fluorescein (2321-07-5,126605-73-0) → 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid 2,5-dioxopyrrolidin-1-yl ester (135795-62-9)

Guidance literature:

With dicyclohexyl-carbodiimide; In N,N-dimethyl-formamide; at 70 - 80 ℃; for 1h; Inert atmosphere;

DOI:10.1002/chem.201300567

Reference yield: 96.0%

6-aminohexanoic acid (60-32-2,93208-38-9) + 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid 2,5-dioxopyrrolidin-1-yl ester (135795-62-9) → 6-[2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-benzoylamino]-hexanoic acid

Guidance literature:

With triethylamine; In water; N,N-dimethyl-formamide; at 20 ℃; for 0.5h;

DOI:10.1021/ac020368+

upstream raw materials:

1-hydroxy-pyrrolidine-2,5-dione

fluorescein free acid

fluorescein

Downstream raw materials:

2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-N,N-dimethylbenzamide

[9-(2-dimethylcarbamoylphenyl)-6-oxo-6H-xanthen-3-yloxy]acetic acid methyl ester

C₂₉H₃₀N₂O₆

4-(4-(2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoyl)piperazin-1-yl)-4-oxo-N-phenylbutanamide

Refernces

• 1、 Huang, Chusen,Yin, Qin,Meng, Jiangjiang,Zhu, Weiping,Yang, Yi,Qian, Xuhong,Xu, Yufang. "Versatile probes for the selective detection of vicinal-dithiol-containing proteins: Design, synthesis, and application in living cells". supporting information. p. 7739 - 7747. Retrieved 2013/07/19.
• 2、 Gao, Jianxin,Wang, Poguang,Giese, Roger W.. "Xanthamide fluorescent dyes". . p. 6397 - 6401. Retrieved 2007/10/03.