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| CAS No.: | 169332-61-0 |
|---|---|
| Name: | AC-DEVD-AMC |
| Molecular Structure: | |
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| Formula: | C30H37 N5 O13 |
| Molecular Weight: | 675.64 |
| Synonyms: | L-a-Asparagine,N2-[N-[N-(N-acetyl-L-a-aspartyl)-L-a-glutamyl]-L-valyl]-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)- |
| Density: | 1.423 g/cm3 |
| Boiling Point: | 1200.1 °C at 760 mmHg |
| Flash Point: | 679.6 °C |
| Solubility: | DMSO: 10 mM |
| Appearance: | white powder |
| PSA: | 287.61000 |
| LogP: | 1.10580 |
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Chemistry
MF: C30H37N5O13
MW: 675.64
Density: 1.423 g/cm3
Storage temp: −20°C
Flash Point: 679.6 °C
Solubility: DMSO: 10 mM
Appearance: white powder
Index of Refraction: 1.594
Enthalpy of Vaporization: 186.47 kJ/mol
Boiling Point: 1200.1 °C at 760 mmHg
Vapour Pressure: 0 mmHg at 25°C
IUPAC Name: 4-[(2-acetamido-4-hydroxy-4-oxobutanoyl)amino]-5-[[1-[[4-hydroxy-1-[(4-methyl-2-oxochromen-7-yl)amino]-1,4-dioxobutan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
Following is the molecular structure of Ac-DEVD-AMC (CAS NO.169332-61-0):
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Uses
The Ac-DEVD-AMC (CAS NO.169332-61-0) substrate was made on a peptide synthesizer and purified according to standard protocols: purity ≥ 98%, MW=675 Daltons.
The Ac-DEVD-AMC (CAS NO.169332-61-0) substrate is routinely 800 evaluated in protease assays using apoptotic and non-apoptotic cell lysates or purified, active Caspase-3. Ac-DEVD-600 AMC has been previously reported to have linear Michaelis-Menton kinetics with a Km of 10 μM for Caspase-3.
The Ac-DEVD-AMC (CAS NO.169332-61-0) fluorogenic substrate is designed to be used in protease assays, like those described in the literature by Mashima et al and Nicholson et al. When Ac-DEVD-AMC is treated with pure and active Caspase-3 or apoptotic cell lysates, AMC is released. AMC release is monitored in a spectrofluorometer at an excitation wavelength of 380 nm and an emission wavelength range of 430-460 nm. Apoptotic cell lysates containing active Caspase-3 yield a considerable emission as compared to non-apoptotic cell lysates. Apoptosis can be induced by a number of mechanisms. For each protease assay, we typically use a 1 ml reaction buffer [20 mM HEPES (pH 7.5), 10% glycerol, 2 mM DTT], 20 μM.
Safety Profile
Safety Information of Ac-DEVD-AMC (CAS NO.169332-61-0):
WGK Germany: 3
Specification
Ac-DEVD-AMC (CAS NO.169332-61-0) is a synthetic tetrapeptide fluorogenic substrate for Caspase-3 (CPP32) and contains the amino acid sequence of the PARP cleavage site at Asp-216. The tetrapeptide substrate can be used to identify and quantify the Caspase-3 activity in apoptotic cells. Caspase-3
cleaves the tetrapeptide between D and AMC, thus releasing the fluorogenic AMC, which can be quantified in a spectrofluorometer. The substrate can also be used to study the inhibition of Caspase-3 by the tetrapeptide aldehyde, Ac-DEVD-CHO or any other inhibitor of Caspase-3.