103780-20-7

Basic information

• Product Name: NOT I
• Synonyms: NOT I;NOT I RESTRICTION ENZYME;RESTRICTION ENDONUCLEASES NOT I;restriction endonuclease not I from*nocardia otid;RESTRICTION ENDONUCLEASE NOT I FROM*NOCA RDIA OTIDIS;NOT I 5'...GC GGCCGC...3' ISOLATED FROM NOCARDIA OTITDIS-CAVIARUM;not i from nocardia otidiscaviarum;RESTRICTION ENDONUCLEASE NOT I
• CAS NO: 103780-20-7
• Molecular Formula: Unspecified
• Molecular Weight: 0
• Product Categories: 3.1.x.x Acting on esters;3.x.x.x Hydrolases;Molecular Biology;Molecular Biology Enzymes;Molecular Biology Tested;Restriction EndonucleasesApplication Index;Restriction EnzymesEnzyme Class Index
• Mol File: 103780-20-7.mol
• Article Data: 0

Chemical Properties

• Appearance: /buffered aqueous glycerol solution
• Storage Temp.: −20°C
• CAS DataBase Reference: NOT I(CAS DataBase Reference)
• NIST Chemistry Reference: NOT I(103780-20-7)
• EPA Substance Registry System: NOT I(103780-20-7)

Safety Data

• RIDADR: UN3373 - class 6.2 Biological substance, Category B
• WGK Germany: 1
• Hazardous Substances Data: 103780-20-7(Hazardous Substances Data)

Usage

Description

NOT I is a rare-cutter enzyme that recognizes the sequence GCGGCCGC and generates fragments with 5′-cohesive termini. It is one of the two known enzymes that recognize an octameric sequence comprised solely of G and C residues. NOT I is used in molecular biology and genetic research for various applications.

Uses

Used in Molecular Biology:
NOT I is used as a restriction enzyme for cutting DNA at specific recognition sites, allowing for precise manipulation and analysis of genetic material.
Used in Genetic Research:
NOT I is employed as a tool in genetic research to study gene function, regulation, and interactions by enabling the isolation and modification of specific DNA sequences.
Used in DNA Cloning:
NOT I is utilized as a cloning enzyme to insert DNA fragments into vectors, facilitating the production of recombinant DNA molecules for various applications in biotechnology and medicine.
Used in DNA Sequencing:
NOT I is applied in DNA sequencing to determine the order of nucleotides in a DNA molecule, providing valuable information for understanding genetic variations and their implications.
Used in Gene Therapy:
NOT I is used as a tool in gene therapy to target and modify specific genes, potentially treating genetic disorders by correcting or replacing faulty genetic material.
Used in Diagnostics:
NOT I is employed in the development of diagnostic tests to detect specific genetic markers associated with diseases, enabling early diagnosis and personalized treatment strategies.