104821-25-2 Usage
Description
DIHYDROETHIDIUM, also known as hydroethidine, is a pink to purple fine crystalline powder that serves as a redox indicator. It exhibits blue fluorescence until oxidized to ethidium, making it a useful tool for detecting superoxide radicals in cells, tissues, and organisms.
Uses
Used in Cellular and Tissue Analysis:
DIHYDROETHIDIUM is used as a redox indicator for detecting superoxide radicals in cells, tissues, and organisms. Its ability to fluoresce upon oxidation allows for the measurement of oxygen reduction in phagocytes during the respiratory burst, providing valuable insights into cellular processes and responses to various stimuli.
Used in Flow Cytometry:
In flow cytometry, DIHYDROETHIDIUM is used as a dye to study various cell types, such as alveolar macrophages, blood leukocytes, and epithelial cells. Its fluorescence properties enable researchers to measure intracellular levels of H2O2 and O2-, as well as other reactive oxygen species, providing a means to assess cellular oxidative stress and other related phenomena.
Used in Reactive Oxygen Species Measurement:
DIHYDROETHIDIUM is used as a probe to measure reactive oxygen species, such as superoxide radicals, in biological systems. Its sensitivity and specificity make it a valuable tool for investigating the role of reactive oxygen species in various physiological and pathological processes.
Check Digit Verification of cas no
The CAS Registry Mumber 104821-25-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,4,8,2 and 1 respectively; the second part has 2 digits, 2 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 104821-25:
(8*1)+(7*0)+(6*4)+(5*8)+(4*2)+(3*1)+(2*2)+(1*5)=92
92 % 10 = 2
So 104821-25-2 is a valid CAS Registry Number.
InChI:InChI=1/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
104821-25-2Relevant articles and documents
Monitoring the Instant Creation of a New Fluorescent Signal for Evaluation of DNA Conformation Based on Intercalation Complex
Uzumcu, Ahmet T.,Guney, Orhan,Okay, Oguz
, p. 1325 - 1332 (2018)
Here we report the monitoring the instant creation of a new fluorescent signal (FS) aroused from a positively charged water-soluble fluorogenic probe, ethidium bromide (EtBr) in the presence of a radical initiator, ammonium persulfate (APS) and an acceler
SYNTHESIS OF ANIONIC AND HYDROPHOBIC DERIVATIVES OF ETHIDIUM BROMIDE AS POTENTIAL NEW TRYPANOCYDES
Loccufier, J.,Schacht, E.
, p. 145 - 152 (2007/10/02)
This paper describes the synthesis of anionic and hydrophobic derivatives of 3,8-diamino-5-ethyl-6-phenyl phenantridium bromide (ethidium bromide, homidium).Evidence for regioselective acylation is provided.
