A bi-enzyme tracer direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed based on anti-imidaclothiz and anti-thiacloprid antibodies. Several affected physicochemical factors were optimised, including methanol concentration, ionic strength and pH value. Under the optimised...detailed
A direct quantum dots-based fluoroimmunoassay (QDFIA) and a time-resolved fluoroimmunoassay (TRFIA) for imidaclothiz (IMI) were developed by using the quantum dots (QDs)-labeled antibody and the europium (Eu3 +)-labeled antibody, respectively. After optimization, the half-maximal inhibition conc...detailed
In this study, we isolated six phage-displayed peptides by biopanning phage-displayed peptide libraries on an immobilized anti-imidaclothiz monoclonal antibody. After analyzing the relative sensitivity of the individual phage-displayed peptides, we subsequently developed and optimized both a pha...detailed
Imidaclothiz, a kind of neonicotinoid insecticides, is developed as a commercial pesticide in China and used for the control of sucking and biting insects, including aphids, whiteflies, beetles and some Lepidoptera species as well. In recent years, more and more attention has been focused on the...detailed
Immunochromatographic strips (ICS) attract much attention as practical analytical tools, particularly for point of care testing. However, ICS suffer from insufficient sensitivity, especially for small molecules. Competitive assays developed with analyte peptidomimetics obtained from phage displa...detailed