106562-32-7 Usage
Description
7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID (AMCA) is a blue fluorescent dye characterized by its relatively large Stoke's shift and resistance to photobleaching. It is a versatile compound used in various applications due to its desirable properties, such as its ability to form photostable amide links with lysine residues upon activation with UV light.
Uses
Used in Fluorescent Labeling:
AMCA is used as a fluorescent protein labeling agent for various applications, including multiplex immunophenotyping. Its N-hydroxysuccinimide ester reacts with lysine residues to form stable amide links, and upon activation with UV light, it displays emission maxima of 400-460 nm.
Used in Contrasting Probes:
In the field of microscopy and imaging, 7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is used as contrasting probes for double and triple labeling in immunofluorescence assays. Its blue fluorescence allows for clear differentiation between multiple markers, enhancing the accuracy and efficiency of these assays.
Used in Non-Activated, Amine-Reactive Fluorescent Probes:
AMCA is also utilized as a non-activated, amine-reactive fluorescent probe in various research and diagnostic applications. Its ability to form stable amide links with lysine residues makes it a valuable tool for detecting and analyzing proteins and other biomolecules.
Check Digit Verification of cas no
The CAS Registry Mumber 106562-32-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,6,5,6 and 2 respectively; the second part has 2 digits, 3 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 106562-32:
(8*1)+(7*0)+(6*6)+(5*5)+(4*6)+(3*2)+(2*3)+(1*2)=107
107 % 10 = 7
So 106562-32-7 is a valid CAS Registry Number.
InChI:InChI=1/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
106562-32-7Relevant articles and documents
Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation
Ou, Pan,Zhang, Ruilong,Liu, Zhengjie,Tian, Xiaohe,Han, Guangmei,Liu, Bianhua,Hu, Zhangjun,Zhang, Zhongping
, p. 2261 - 2265 (2019)
Enzyme activity in live cells is dynamically regulated by small-molecule transmitters for maintaining normal physiological functions. A few probes have been devised to measure intracellular enzyme activities by fluorescent imaging, but the study of the re
A labeled guanidine ligand for studying sweet taste
Feng, Yangbo,Burgess, Kevin,Pledger, David,Cairns, Nick,Linthicum, D. Scott
, p. 881 - 884 (2007/10/03)
A synthesis of a biotinylated-, coumarin-substituted-N,N'-diarylguanidin 1 is reported. This ligand has structural features conducive to studying sweet taste including a fluorescent tag to facilitate spectroscopic studies of binding to protein-receptors f
