111072-31-2 Usage
Description
XTT sodium salt, also known as sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate, is a tetrazolium salt that forms water-soluble formazan on bioreduction. It is a valuable tool in various research and testing applications due to its ability to indicate cell viability and proliferation.
Uses
Used in Cancer Research:
XTT sodium salt is used as a screening agent for human cancer cell lines, in conjunction with phenazine methosulfate. It aids in the study of fungal cell damage and is involved in testing the antimicrobial susceptibility of staphylococci. Additionally, it is utilized in candida biofilm research, contributing to a better understanding of these organisms and their interactions with antimicrobial agents.
Used in AIDS and Cancer Testing:
XTT sodium salt serves as a vital component in assays for cell viability and cell proliferation, which are crucial for evaluating the effects of potential treatments in both AIDS and cancer research. Its ability to form water-soluble formazan upon bioreduction allows for accurate and reliable measurements of cell activity, making it an indispensable tool in these fields.
Used in Antimicrobial Susceptibility Testing:
In the field of microbiology, XTT sodium salt is used to test the susceptibility of various microorganisms, particularly staphylococci, to antimicrobial agents. This helps in determining the effectiveness of different treatments and contributes to the development of new antibiotics and therapeutic strategies.
Used in Fungal Research:
XTT sodium salt plays a significant role in studying fungal cell damage, which is essential for understanding the mechanisms of action of antifungal agents and developing new treatments for fungal infections.
Used in Biofilm Research:
In the study of biofilms, particularly candida biofilms, XTT sodium salt is employed to assess the viability and proliferation of cells within these complex structures. This knowledge is crucial for developing strategies to combat biofilm-associated infections, which are often resistant to conventional treatments.
Biochem/physiol Actions
XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.
Check Digit Verification of cas no
The CAS Registry Mumber 111072-31-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,1,0,7 and 2 respectively; the second part has 2 digits, 3 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 111072-31:
(8*1)+(7*1)+(6*1)+(5*0)+(4*7)+(3*2)+(2*3)+(1*1)=62
62 % 10 = 2
So 111072-31-2 is a valid CAS Registry Number.
InChI:InChI=1/C22H19N7O13S2.2Na.H2O/c1-41-18-8-16(28(31)32)20(43(35,36)37)10-14(18)26-12-25(22(30)23-13-6-4-3-5-7-13)24-27(26)15-11-21(44(38,39)40)17(29(33)34)9-19(15)42-2;;;/h3-12H,1-2H3,(H,23,30)(H,35,36,37)(H,38,39,40);;;1H2/q+2;2*+1;/p-2
111072-31-2Relevant articles and documents
The Synthesis of XTT: A New Tetrazolium Reagent that is Bioreducible to a Water-Soluble Formazan
Paull, Keneth D.,Shoemaker, Robert H.,Boyd, Michael R.,Parsons, Jack L.,Risbood, Prabhakar A.,et al.
, p. 911 - 914 (2007/10/02)
A new tetrazolium salt, XTT, has been synthesized.XTT is reduced by a considerable variety of cell lines to a water-soluble formazan.XTT appears to merit further investigation as a reagent for broader application to cell culture assay systems.
